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  • 1
    Electronic Resource
    Electronic Resource
    The use of fluorescent in situ hybridization for detection of the t(2;5)(p23;q35) translocation in anaplastic large-cell lymphoma (1997)
    Springer
    Annals of oncology 8 (1997), S. 65-69 
    Details
    ISSN: 1569-8041
    Keywords: anaplastic large-cell lymphoma ; fluorescent in situ hybridization ; Hodgkin's disease ; Ki-1 lymphoma ; PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background: Anaplastic large-cell lymphoma (ALCL) is a recently recognized, distinctive type of non-Hodgkin's lymphoma characterized by anaplastic large-cell cytology and expression of a member of the TNF-receptor family CD30. A characteristic chromosomal translocation has been identified in ALCL of T- or null-cell lineage which juxtaposes a novel tyrosine kinase (anaplastic lymphoma kinase, ALK) located at 2p23 with the nucleophosmin gene (NPM) at 5q35. A chimeric mRNA transcript is produced, and the translocation results in constitutive expression of a truncated form of the ALK protein, p80. There is controversy concerning whether or not the translocation occurs in Hodgkin's disease. The aim of this study was to develop a methodology for fluorescent in situ hybridization (FISH) to detect the t(2;5)(p23;q35), and to compare the results with conventional cytogenetics, reverse-transcriptase PCR and immunostaining for the p80protein. Patients and methods: Twenty-five cases of malignant lymphoma (11 ALCL and 14 HD) were studied. Immunohistochemistry was performed to confirm the diagnosis and for analysis of p80 expression. Conventional cytogenetics were analyzed on G-banded metaphase spreads. FISH was performed using whole chromosome paints for chromosomes 2 and 5 on metaphase spreads and YAC probes for inter phase nuclei. Reverse-transcriptase PCR using primers for ALK and NPM was used to amplify the translocation breakpoint in extracted mRNA. Results: Among 11 cases of ALCL examined by FISH, the translocation was detected in 4. Two of these cases also had RT-PCR and p80 staining performed, with positive results. Among 7 cases where the t(2;5) was not detected by FISH, 3 cases were examined by RT-PCR with negative results and4 cases by p80 staining, also negative. The RT-PCR was negative in all 14cases of Hodgkin's disease, 4 of which were also examined by FISH and found to be negative. Conclusion: Fluorescent in situ hybridization is useful method for detection of the t(2;5)(p23;q35) in anaplastic large-cell lymphoma. The results concur with those of RT-PCR for the chimeric transcript and immunostaining for the p80 protein. The frequency with which the translocation was found was 36% in this small series, and no evidence of the translocation was found in cases of Hodgkin's disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008234301024
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  • 2
    Electronic Resource
    Electronic Resource
    The use of fluorescent in situ hybridization for detection of the t(2;5)(p23;q35) translocation in anaplastic large-cell lymphoma (1997)
    Springer
    Annals of oncology 8 (1997), S. 65-69 
    Details
    ISSN: 1569-8041
    Keywords: anaplastic large-cell lymphoma ; fluorescent in situ hybridization ; Hodgkin's disease ; Ki-1 lymphoma ; PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background: Anaplastic large-cell lymphoma (ALCL) is a recentlyrecognized, distinctive type of non-Hodgkin's lymphoma characterized byanaplastic large-cell cytology and expression of a member of theTNF-receptor family CD30. A characteristic chromosomal translocation hasbeen identified in ALCL of T- or null-cell lineage which juxtaposes a noveltyrosine kinase (anaplastic lymphoma kinase, ALK) located at 2p23 with thenucleophosmin gene (NPM) at 5q35. A chimeric mRNA transcript is produced,and the translocation results in constitutive expression of a truncated formof the ALK protein, p80. There is controversy concerning whether or not thetranslocation occurs in Hodgkin's disease. The aim of this study was todevelop a methodology for fluorescent in situ hybridization (FISH) to detectthe t(2;5)(p23;q35), and to compare the results with conventionalcytogenetics, reverse-transcriptase PCR and immunostaining for the p80protein. Patients and methods: Twenty-five cases of malignant lymphoma (11 ALCLand 14 HD) were studied. Immunohistochemistry was performed to confirm thediagnosis and for analysis of p80 expression. Conventional cytogenetics wereanalyzed on G-banded metaphase spreads. FISH was performed using wholechromosome paints for chromosomes 2 and 5 on metaphase spreads and YACprobes for interphase nuclei. Reverse-transcriptase PCR using primers forALK and NPM was used to amplify the translocation breakpoint in extractedmRNA. Results: Among 11 cases of ALCL examined by FISH, the translocation wasdetected in 4. Two of these cases also had RT-PCR and p80 stainingperformed, with positive results. Among 7 cases where the t(2;5) was notdetected by FISH, 3 cases were examined by RT-PCR with negative results and4 cases by p80 staining, also negative. The RT-PCR was negative in all 14cases of Hodgkin's disease, 4 of which were also examined by FISH and foundto be negative. Conclusion: Fluorescent in situ hybridization is useful methodfor detection of the t(2;5)(p23;q35) in anaplastic large-cell lymphoma. Theresults concur with those of RT-PCR for the chimeric transcript andimmunostaining for the p80 protein. The frequency with which the translocationwas found was 36% in this small series, and no evidence of thetranslocation was found in cases of Hodgkin's disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008234301024
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  • 3
    Electronic Resource
    Electronic Resource
    Fluorescence depolarization studies of conformation changes in pyrene-butyryl-fumarase (1978)
    New York : Wiley-Blackwell
    Biopolymers 17 (1978), S. 2373-2384 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Measurements of fluorescence depolarization on fumarase labeled with the dye pyrene-butyryl were used to test for previously reported structural changes in this enzymes. These apparent conformation changes were of interest because they seemed to correlate with variation in catalytic activity provoked by changing temperature or pH, or by the presence of a competitive inhibitor. In the present studies, the bound dye pyrene-butyryl and the enzymes were investigated systematically to ensure that simple interpretation of fluorescence depolarization results would be meaningful. This analysis showed that carefully controlled experimental condition were necessary to eliminate a dye component with a short fluorescence lifetime and that it was essential to allow for small variations of lifetime with temperature. Contrary to the previous report, a constant rotational relaxation time of the magnitude expected for a nearly spherical molecule of fumarase was found. No changes were detectable by fluorescence depolarization in the size or shape of pyrene-butyryl-fumarase under the solution conditions tested that caused variation in enzyme activity.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.1978.360171007
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  • 4
    Electronic Resource
    Electronic Resource
    Novel curing systems for chlorosulfonated polyethylene (1964)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 8 (1964), S. 337-354 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Polyfunctional alcohols, acyclic, cyclic, and heterocyclic imines, and organotin oxides have been studied as crosslinking agents for chlorosulfonated polyethylene. Mechanisms for the crosslinking reactions involving the sulfonyl chloride moiety have been proposed. Differences in curing activity have been correlated with structural modifications of the curing agents consistent with classical chemical reactions for nonpolymeric systems. Of the curing systems examined, the polyols provide nontoxic, low cost, safe-processing formulations offering excellent physical properties which have found use in practical commercial applications.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1964.070080121
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  • 5
    Electronic Resource
    Electronic Resource
    A general correlation of the flammability of natural and synthetic polymersPresented at the 3rd International Cellular Plastics Conference, The Society of the Plastics Industry, Montreal, September 27, 1972. (1974)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 18 (1974), S. 491-504 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The concept that flammability is fundamentally related to the potential thermal energy available per unit of volume of material emerged from attempts to correlate the effect of composition variables on the flammability of neoprene vulcanizates as measured by the oxygen index (O.I.) test. The origins of this test clearly show that it is a highly specific measure of flammability - the tendency of a composition to continue to burn once ignited - and that it is thermodynamically related to the heat of combustion of materials. This relationship is developed to a linear correlation which includes a wide variety of synthetic and natural materials and permits reasonable prediction of O.I. values from elemental analysis. Polymeric materials containing carbon and oxygen in atom ratios of less than 6 to 1 are more flammable than predicted. The effect of atmospheric temperature on O.I. can be predicted in relation to the O.I. value at normal temperature. This effect is shown to be independent of the composition of the material being tested. These two correlations permit the construction of a simple general map of flammability against which experimental data can be compared and judgments made with respect to the significant variables involved. There appears to be a significant relation between O.I. data, as viewed from these correlations, and the data of other flammability tests.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1974.070180215
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  • 6
    Electronic Resource
    Electronic Resource
    An in vitro and in vivo analysis of anodized tantalum capacitive electrodes: Corrosion response, physiology, and histology (1977)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 11 (1977), S. 637-656 
    Details
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Oxidation-reduction reactions which can destroy high current-density metalstimulating electrodes are avoided when using capacitive electrodes. The results of in vitro and in vivo testing of anodized, high surface area, sintered tantalum electrodes are presented. The corrosion response of the electrodes is excellent; there is no evidence of dissolution of the electrode. A deposit forms on the surface of the electrodes, but has little effect on the voltage response to constant current stimulation. The physiological and histopathological results indicate the capacitive tantalum electrode to be the safest yet tested.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbm.820110502
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  • 7
    Electronic Resource
    Electronic Resource
    An in vitro model for evaluating neural stimulating electrodes (1976)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 10 (1976), S. 907-928 
    Details
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: A model for in vitro evaluation of materials for use as neural stimulation electrodes is developed. Critical areas of concern in developing an in vitro test model discussed include: selection of environment, choice of material, design of stimulating equipment, and analytical procedures used to evaluate materials response. A method of providing quantitative analysis of materials response to stimulation conditions is presented. Evaluation techniques involve the use of scanning electron microscopy, x-ray spectroscopy, atomic absorption spectrometry, and potentiographic and dielectric analysis of the test electrodes. A diagnostics matrix is presented which assigns a scale factor to quantify the relative corrosion response of the candidate materials. The corrosion response of Pt electrodes is evaluated in terms of scale factors assigned through use of the diagnostics matrix.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbm.820100609
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  • 8
    Electronic Resource
    Electronic Resource
    Ultracentrifuge studies on the crystalline compound of trypsin and of soybean trypsin inhibitor (1950)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Polymer Science 5 (1950), S. 375-375 
    Details
    ISSN: 0022-3832
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pol.1950.120050308
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  • 9
    Electronic Resource
    Electronic Resource
    On the interaction of the globulin, arachin, with various ionsPresented at the International Congress of Pure and Applied Chemistry, New York, September, 1951. (1951)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Polymer Science 7 (1951), S. 605-634 
    Details
    ISSN: 0022-3832
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Additional Material: 18 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pol.1951.120070607
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  • 10
    Electronic Resource
    Electronic Resource
    Investigations on cellulose nitrate. II. Liquid and vapor phase adsorption of acetonePart I of this series appeared in J. Polymer Sci., 3, 735 (1948). (1949)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Polymer Science 4 (1949), S. 247-263 
    Details
    ISSN: 0022-3832
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The discrepancies in the published work on the interaction of acetone and cellulose nitrate are discussed and explained. Vapor phase adsorption isotherms for the adsorption of acetone by cellulose nitrate have been determined with an adsorption balance. They have been shown to be independent of the crystalline-amorphous ratio of the polymer but dependent upon its nitrogen content. In cellulose nitrates which are completely soluble in acetone, no limiting adsorption has been found. Adsorption analysis (a modification of chromatography) has been applied to the determination of the liquid phase adsorption by cellulose nitrate of acetone from mixtures with petroleum ether. In terms of the liquid mixture concentrations, there appears to be limited adsorption, but this is shown to be due to the nonideal nature of the solutions of acetone and petroleum ether. When allowance is made for this, the liquid and vapor phase isotherms are the same within experimental error and do not support the existence of compounds of acetone and cellulose nitrate.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pol.1949.120040302
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