ZIB

Hits per page

hit 1 - 1 | 1 hit

Sorting

Electronic Resource

Fusion polypeptides in gene cloning: Potential problems due to conformational alterations at the junction (1987)

Querol, E. ; Parrilla, A.

Springer

Cellular and molecular life sciences 43 (1987), S. 920-922

add to mindlist on the mindlist

Details

ISSN: 1420-9071

Keywords: Molecular cloning ; fusion polypeptides ; protein conformation ; protein folding

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Summary Many eukaryotic genes are cloned in bacterial hosts as fusion polypeptides. Prediction of the secondary structures for some common prokaryotic fusion polypeptides shows that many junction sites correspond to important secondary structures. It is suggested that such structures could affect (hinder, etc.) the conformation or drive the folding of the neighboring eukaryotic counterparts. Thus the prokaryotic junction should be better performed, in random coil regions, or short fusion prokaryotic polypeptides should be sued.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01951668

Permalink

Library	Location	Call Number	Volume/Issue/Year	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

hit 1 - 1 | 1 hit