ISSN:
1573-4986
Keywords:
Ehrlich tumour cells
;
galactosyl residues
;
Griffonia simplicifolia I-B4 isolectin
;
lectin-gold
;
electron
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract We have studied by high resolutionin situ light and electron microscopic lectin-gold techniques the subcellular distribution of α-d-Gal residues using theGriffonia simplicifolia I-B4 isolectin and compared it with that of β-d-Gal residues as detected with theDatura stramonium lectin in Ehrlich tumour cells grown as ascites or monolayer. The microvillar but not the smooth plasma membrane regions were labelled with theGriffonia simplicifolia I-B4 isolectin whereas both plasma membrane regions were equally well labelled with theDatura stramonium lectin. Elements of the endocytotic/lysosomal system such as coated membrane invaginations and vesicles, early and late endosomes and secondary lysosomes were positive for both α-d-Gal and β-d-Gal residues. A particular feature of Ehrlich tumour cells is an elaborate tubular membrane system located in the pericentriolar region which is labelled throughout by both lectins and represents part of the endosomal system. In the Golgi apparatus labelling with both lectins was observed to commence in trans cisternae which is indirect evidence for a joint distribution of the sequentially acting β1,4 and α1,3-galactosyl-transferases.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00731358
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