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  • 1
    Electronic Resource
    Electronic Resource
    Instability of phenotype and gene expression in long-term culture of carrot hairy root clones (1999)
    Springer
    Plant cell reports 19 (1999), S. 43-50 
    Details
    ISSN: 1432-203X
    Keywords: Key words Agrobacterium rhizogenes ; β-Glucuronidase ; Daucus carota ; gus gene ; Root culture ; NAM test ; Opines ; Gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Hairy root clones were established from carrot root discs inoculated with an agropine-type strain of Agrobacterium rhizogenes A4 harbouring the gus reporter gene on the TL-DNA. The clones were periodically examined for their phenotypic characteristics and for their ability to express the gus gene, to produce opines and to grow in the presence of NAM. The presence of the gus gene in the roots was confirmed by Southern blot hybridisation. The clones displayed various morphologies which were generally not correlated with the transformation events, and they were highly unstable throughout the successive subcultures, both for their phenotype and for their ability to express the transgenes. Reversible inactivation of the gus gene expression was associated with a high gus copy number. This could have some consequences for fundamental studies and practical uses of hairy roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050708
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The pleiotropic effects induced by therolC gene in transgenic plants are caused by expression restricted to protophloem and companion cells (1996)
    Springer
    Transgenic research 5 (1996), S. 3-11 
    Details
    ISSN: 1573-9368
    Keywords: Agrobacterium rhizogenes ; gene expression ; GUS staining ; immunolocalization ; Nicotiana tabacum ; rolC gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Expression of therolC gene fromAgrobacterium rhizogenes causes morphological and developmental alterations in transgenic plants. The histological alterations underlying the macroscopic changes and the cellular localization of the site of expression of therolC gene have shown that: (i) the expression of therolC gene is developmentally regulated, (ii) in vegetative transgenic plants, the expression of therolC gene under the control of its own promoter is restricted to companion and protophloem cells, (iii) the site of action of the product(s) of the activity of the rolC enzyme is distinct from its site of expression, (iv) precise localization of the rolC peptide has been achieved by immunocytochemistry but not by the histochemical GUS assay. These results imply that the sites of action and expression of therolC gene in trangenic plants are physically separated. Thus the product(s) of the activity of the rolC enzyme must be a factor capable of being transported. Current models forrolC gene action are discussed taking into account the reported results.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01979917
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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