ISSN:
1573-3017
Keywords:
biomarkers
;
heavy metal combinations
;
hsp70
;
hsp60
;
isopods
;
stress proteins
Source:
Springer Online Journal Archives 1860-2000
Topics:
Energy, Environment Protection, Nuclear Power Engineering
Notes:
Abstract The induction of stress-70 (hsp70) and stress-60 (hsp60, chaperonin 60) in the terrestrial woodlouse, Oniscus asellus, after exposure to various heavy metals and heavy metal combinations was investigated by means of standard Western-blot using monoclonal antibodies. The expression of stress-70 was found to react much more sensitively to metal contamination than that of stress-60. The general course of the stress-70 response to increasing metal concentration was found to be similar for all treatments. Based on the obtained results, this course could be divided into three zones: in the first zone, the expression of stress-70 proteins was only marginally increased by comparatively low metal concentrations; in the second zone, strong induction of stress-70 occurred and the ability of the heat shock system to react to increased metal concentrations reached its climax; and in the third, non-compensation zone, a further increase in heavy metal concentration resulted in a decline of stress-70 levels, which can be interpreted as a result of pathological tissue damage. Combinations of metals were found to increase the stress- 70 level more intensely than related concentrations of singularly applied metals. Based on these studies, a ranking scheme of increasing toxicological impact of a variety of heavy metal combinations was composed
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1018682928839
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