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Digitale Medien

Hydroxylation in isolated liver cells (1975)

Kanaeva, I. P. ; Archakov, A. I.

Springer

Bulletin of experimental biology and medicine 80 (1975), S. 1193-1194

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Details

ISSN: 1573-8221

Schlagwort(e): isolated cells ; hydroxylation ; oxidative phosphorylation ; oxidation

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Abstract Isolated cells were obtained by using EDTA and mechanical action to dissociate rat liver tissue. The presence of oxidative phosphorylation reactions in the hepatocytes was taken as an indication of their integrity. The hepatocytes were able to hydroxylate dimethylaniline, ethylmorphine, and aminopyrine. The maximal velocity of hydroxylation was higher in the cells than in microsomes, when calculated per nmole cytochrome P-450. The NAD·H2 formed by oxidation of glutamate and malate in isolated cells can be used for the hydroxylation reactions.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00833157

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