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  • 1
    Electronic Resource
    Electronic Resource
    Gene transfer into insect brain and cell-specific expression of bombyxin gene (1999)
    Springer
    Development genes and evolution 209 (1999), S. 447-450 
    Details
    ISSN: 1432-041X
    Keywords: Key words Bombyxin ; Insulin ; Bombyx mori ; Electroporation ; Cell-specific expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A transgene reporter consisting of the bombyxin gene promoter and the green fluorescent protein coding region was introduced into intact brains of the silkworm Bombyx mori by in vitro electroporation. After in vitro culture of the brains, the fluorescence derived from the introduced reporter gene was observed in all cases in eight neurosecretory cells that had previously been identified as bombyxin-producing cells (BPCs). Although the fluorescence was not always observed in all cells, it was specific to BPCs, indicating that the reporter was under the control of the bombyxin gene promoter in a BPC-specific manner. Electroporatical introduction of a reporter gene was therefore found to be a suitable method for analyzing cell-specific expression in intact tissues and to be substitute for germ-line transmission of reporters in the transgenic system. Application of this technique enables us to analyze the cell-specific expression of transgene reporters within a few days and treat more than several dozens of the reporters within 1 month, which is difficult to do with the transgenic system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004270050277
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Influence functions for stress and displacement discontinuity elements in an anisotropic medium (1997)
    New York, NY [u.a.] : Wiley-Blackwell
    International Journal for Numerical and Analytical Methods in Geomechanics 21 (1997), S. 635-652 
    Details
    ISSN: 0363-9061
    Keywords: anisotropic elastic medium ; complete plane-strain problem ; influence function ; stress discontinuity element ; displacement discontinuity element ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Geosciences
    Notes: Influence functions, that permit us to determine stresses and displacements at an arbitrary point in an infinite, homogeneous, linear elastic, anisotropic medium due to different three-dimensional (3-D) stress or displacement discontinuities distributed on infinite, flat, band-type elements, are presented. Any straight-line segment on the band, which is perpendicular to its infinite side, has the same distribution of the discontinuities. Along with the functions, their Taylor series approximations are also provided. The last can be useful to analyse stresses and displacements at points distant from the elements. The functions allow us to avoid procedures of numerical integration in the Indirect Boundary Element Method and/or the Displacement Discontinuity Method computer codes that are able to solve complete plane-strain problems with 3-D boundary conditions for an elastic, anisotropic medium. © 1997 by John Wiley & Sons, Ltd.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
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