ISSN:
1435-1803
Keywords:
malondialdehyde
;
tetramethoxypropane
;
thiobarbituric acid
;
fatty acids
;
isolated Langendorff's and working
;
rat hearts
;
intact working swine hearts
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Toxic peroxides result when oxygen radicals with various peroxidic precursors in tissue or blood. A colorimetric method based on reactions of malondialdehyde (MDA), a key intermediate in the formation of peroxides, has been described in fresh tissues. The present report adapts this assay to measure MDA levels in frozen heart muscle from rats and swine. Mean tissue values of MDA ranged from 154–353 nmol/g (n=13) in fresh rat hearts perfused by the Langendorff technique. Values in frozen samples were 228±14 nmol MDA/g (n=22). When mechanical function was increased in isolated working rat hearts, tissue MDA levels decreased by-25
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01910480
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