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  • 1
    Electronic Resource
    Electronic Resource
    Failure of successful intrasplenic transplantation of islets from lean mice to cure obese-hyperglycaemic mice, despite islet growth (1981)
    Springer
    Diabetologia 20 (1981), S. 237-241 
    Details
    ISSN: 1432-0428
    Keywords: Obese-hyperglycaemic mice ; isolated pancreatic islets ; islet transplantation ; serum glucose ; islet volume ; autoradiography ; islet cell replication ; immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Implantation of allogeneic pancreatic islets encapsulated in Millipore diffusion chambers has been reported to normalize the obese-hyperglycaemic syndrome in mice. In the present study, both young and adult ob/ob mice remained hyperglycaemic and gained weight after intrasplenic implantation of 500 isogeneic islets isolated from lean mice. Such islets normalized the elevated blood-glucose of alloxan-diabetic lean mice. Morphometric analysis of the intrasplenically implanted islets showed that the mean islet volume in the ob/ob mice was five times larger than that of the lean, non-diabetic mice. Immunocytochemical staining of the spleens showed an increased proportion of B-cells in the enlarged, intrasplenic islets in the ob/ob mice. Moreover, autoradiographical examination of these islets demonstrated the presence of several labelled cells. These results suggest that the growth of the implanted “lean” islets is due to extrapancreatic factors which stimulate islet cell replication in the obese-hyperglycaemic mouse.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00252634
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The renal subcapsular site offers better growth conditions for transplanted mouse pancreatic islet cells than the liver or spleen (1986)
    Springer
    Diabetologia 29 (1986), S. 670-672 
    Details
    ISSN: 1432-0428
    Keywords: Mouse panreatic islets ; islet transplantation ; implantation sites ; autoradiography ; labelling index ; islet cell replication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In order to investigate the importance of the transplantation site for the replication of grafted islet cells, we implanted syngeneic mouse pancreatic islets intrasplenically, intraportally and subcapsularly in the kidney. Fourteen days later the alloxan-diabetic mice were killed after an injection of tritiated thymidine, and the graft-bearing organs fixed and autoradiography. The highest labelling indices were recorded for subcapsularly grafted islets, followed by intraportal and intrasplenic islets in that order. In separate experiments some islet-containing kidney sections were immune stained for insulin before the autoradiographic process. The labelling index of the insulin-positive cells was as high as in the entire islet cell population of the sections from the same mice stained with haematoxylin only. This indicates that the B cells of the islets replicate as often as the other islet cell types. The present data also suggest that the renal subcapsular space offers better growth conditions for transplanted islet cells than the liver or spleen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00869269
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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