ISSN:
1040-7685
Keywords:
micellar electrokinetic capillary chromatography
;
capillary electrophoresis
;
isotopic separations
;
micellar separations
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Near-baseline resolution of dansylated methylamine and dansylated methyld3-amine can be achieved by micellar electrokinetic capillary chromatography (MECC) utilizing a 25-mM sodium dodecylsulfate pseudostationary phase and a mobile phase containing 4.9 M methanol. This study examines the role of methanol on this separa- tion by looking at its effects on to, tr,tm, and resolution over four methanol concentrations, 0 M, 2.5 M, 4.9 M, and 7.4 M. The 4.9 M methanol buffer proves to be the best for optimizing the resolution of these two extremely similar compounds. Because of the high organic content of the mobile phase, it is unreliable to determine tm based upon the migration time of a single species. A method for determining tm based upon a homologous series of standards is presented and examined over the four methanol concentrations. Plots of log k′ versus carbon number yield straight lines with similar slopes for each of the four buffer systems, indicating that this procedure is a good method for tm determination.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/mcs.1220010304
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