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Digitale Medien

Novel and sensitive noncompetitive enzyme immunoassay for kassinin in rat plasma (1989)

Tanaka, K. ; Hashida, S. ; Kohno, T. ; [weitere]

Springer

Cellular and molecular life sciences 45 (1989), S. 470-472

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Details

ISSN: 1420-9071

Schlagwort(e): Enzyme immunoassay ; peptide ; kassinin ; biotinylation

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Summary A novel and sensitive noncompetitive enzyme immunoassay for kassinin is described. Kassinin was biotinylated using sulfosuccinimidyl-6-(biotinamido)hexanoate. The biotinylated kassinin was trapped on antikassinin IgG-coated polystyrene balls and, after washing to eliminate other biotinylated substances, was eluted with HCl. The biotinylated kassinin eluted was reacted with anti-kassinin Fab'-peroxidase conjugate and trapped onto streptavidin-coated polystyrene balls. Peroxidase activity bound to the polystyrene balls was assayed by fluorimetry. The detection limit of kassinin was 0.13 pg (0.1 fmol)/tube or 0.065 μg/l of rat plasma, which was 750-fold or 15-fold lower than that for competitive radioimmunoassay.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF01952033

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