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  • 1
    Electronic Resource
    Electronic Resource
    Dietary spermine supplementation induces intestinal maturation in sea bass (Dicentrarchus labrax) larvae (1997)
    Springer
    Fish physiology and biochemistry 16 (1997), S. 479-485 
    Details
    ISSN: 1573-5168
    Keywords: polyamine ; spermine ; sea bass ; larvae ; intestinal maturation ; pancreatic enzymes ; intestinal enzymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sea bass (Dicentrarchus labrax) larvae were fed microparticulated compound diet containing 0 (FP0), 0.10 (FP10) and 0.33% (FP33) of a polyamine, spermine, from day 20 to day 38. LP group was fed live prey. This group exhibited the highest growth and survival. The addition of spermine did not lead to growth enhancement. A 33% survival improvement was obtained in FP33 group compared to FP0 group. The spermine addition affected the activity of pancreatic enzymes, trypsin, chymotrypsin and amylase, during larvae development. This non specific effect suggested that the action of spermine would be mediated by hormones. In the intestine, the FP33 group exhibited from day 31 higher activities of brush border membrane enzymes (leucine aminopeptidase and alkaline phosphatase) and lower level in a cytosolic enzyme (leucine-alanine peptidase) compared to FP10 and FP0 group. The diet containing the highest spermine level induced an enzymatic profile similar to that obtained in LP group and characteristic of a mature enterocyte. The initiation of enterocyte maturation at a proper development stage was associated to the survival improvement observed in FP33 group.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007786128254
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Dietary regulation of activities and mRNA levels of trypsin and amylase in sea bass (Dicentrarchus labrax) larvae (1998)
    Springer
    Fish physiology and biochemistry 19 (1998), S. 145-152 
    Details
    ISSN: 1573-5168
    Keywords: sea bass ; larvae ; dietary adaptation ; gene regulation ; trypsin ; amylase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Specific activities and mRNA levels of trypsin and amylase were studied in sea bass larvae. From day 20 to day 40, Dicentrarchus labrax were fed two rations of one day old Artemia: satiation (LP) and one-eighth of the satiation ration (LP/8) or two isoenergetic compound diets that varied in protein (30 and 60%) and carbohydrate (37 and 7%) content (FP30 and FP60 respectively). Trypsin mRNA levels and specific activities were mainly influenced by the nature of dietary protein and the Artemia ration. By using fish meal as protein source, dietary protein concentration did not affect either mRNA level nor specific activity of trypsin. These results suggested that the trypsin synthesis was not affected at a transcriptional level by the protein ration, i.e., Artemia ration. Decrease in amylase mRNA observed from day 29 in the four dietary groups suggested that this decrease in amylase expression is genetically programmed during sea bass larvae development. Nevertheless, the composition and the quantity of the diet influenced the amylase specific activities revealing primarily translational regulation of amylase. This study shows for the first time that the molecular mechanisms which control the dietary adaptation of trypsin and amylase are independently regulated, age-dependent and influenced by the composition and the quantity of the diet.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007775501340
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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