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  • 1
    Electronic Resource
    Electronic Resource
    Cell-free translation of exogenous mRNA in extracts from dry pea primary axes (1980)
    Springer
    Planta 147 (1980), S. 302-306 
    Details
    ISSN: 1432-2048
    Keywords: mRNA ; Protein synthesis ; Pisum ; Seeds (translation) ; Translation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Extracts from the primary axes of dry pea (Pisum sativum L.) seeds are able to perform an initiation-dependent translation of exogenous mRNA. SDS polyacrylamide gel electrophoresis of the products synthesized under direction of alfalfa mosaic virus RNA (AMV-RNA) and tobacco mosaic virus RNA (TMV-RNA) shows that the fidelity of translation in this pea system is at least as high as in a wheat embryo cell-free protein synthesizing system. The endogenous messengers are also efficiently translated in extracts from the primary axes of pea seeds. The direct translation of these messengers in a homologous cell-free system may be of interest for a study of the products coded for by the long-lived messengers present in this plant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00379837
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization of a maturation-specific mRNA in dry mung bean embryonic axes (1980)
    Springer
    Planta 149 (1980), S. 227-233 
    Details
    ISSN: 1432-2048
    Keywords: Germination (seeds) ; Immunoprecipitation ; mRNA ; Poly(A) RNA ; Vigna
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The poly(A)-rich RNA from dry mung bean (Vigna radiata [L.] Wilczek) embryonic axes has been isolated and translated in a wheat embryo cell-free system, and the products were analyzed on sodium dodecyl sulfate-polyacrylamide gels. The fluorographyic patterns showed a heavy band at approximately MW 12,000. The messenger RNA coding for this polypeptide disappeared in the course of early germination. This messenger is translated in vivo but simultaneously degrades when the axes imbibe. The poly(A)-rich RNA from dry axes has been fractionated on sucrose-dimethyl sulfoxide gradients, and this messenger has been found to be distributed largely in the 9–14 S region. The polypeptide synthesized in vitro has been immunoprecipitated, using the antiserum raised against this protein purified from dry axes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384558
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Some aspects of the synthesis of long-lived messenger ribonucleoproteins in developing rye embryos (1979)
    Springer
    Planta 144 (1979), S. 485-490 
    Details
    ISSN: 1432-2048
    Keywords: Embryo (mRNA) ; Messenger ribonucleoprotein ; mRNA ; Ribonucleoprotein (messenger) ; Secale ; Seed development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Untranslated messenger ribonucleoproteins (mRNPs informosomes) are present in developing rye (Secale cereale, L. cv. Celestijner) embryos throughout the last 5 weeks of seed formation. Ribosomal as well as non-ribosomal ribonucleoproteins are formed continuously both in primary axes and scutella until any synthesis of macromolecules stops upon dessication. The content of preformed messengers in the primary axes of precociously harvested rye grains increases as a function of embryo development. This increase of the “template load” of the primary axes results from a continuous accumulation of qualitatively identical mRNPs. Germination experiments demonstrated that at least most of the preformed messengers are not required for the germination process. Their function is discussed in terms of selective adaptation to unfavorable conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00380127
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Preformed and newly synthesized messenger RNA in germinating wheat embryos (1979)
    Springer
    Planta 144 (1979), S. 491-496 
    Details
    ISSN: 1432-2048
    Keywords: Embryo (mRNA) ; Germination (embryo) ; Messenger ribonucleoprotein ; mRNA ; Ribonucleoprotein (messenger) ; Translation ; Triticum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In 6 h germinated wheat (Triticum aestivum L. cv. Cama) embryos, more than half of the messenger RNAs are actively involved in translation. Neither preformed nor newly synthesized poly A+-RNA is translated preferentially. Germination in the presence of cordycepin showed that the half-life of the templates is about 2 h and that the newly synthesized messengers are essential to support protein synthesis in the embryo from the first hours of germination. Most of the messenger RNAs in 6 h germinated embryos are newly synthesized. The polypeptides coded for by either the endogenous messenger ribonucleoproteins or purified poly A+-RNA from both dry and germinated embryos are qualitatively identical; minor quantitative differences can however be observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00380128
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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