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Frequency of apoptosis relates inversely to invasiveness and metastatic activity in human colorectal cancer (1997)

Hashimoto, Satoshi ; Koji, Takehiko ; Kohara, Norihiro ; [et al.]

Springer

Virchows Archiv 431 (1997), S. 241-248

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ISSN: 1432-2307

Keywords: Key words Colorectal cancer ; Apoptosis ; Cell differentiation ; Tumour invasiveness ; Metastasis

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract The frequency of apoptosis was determined in 102 cases of human colorectal cancer. The results were correlated with the frequency of cell proliferation and with clinicopathological characteristics such as degree of differentiation, invasiveness and metastasis. As a marker of apoptosis, intranuclear DNA strand breaks were localized with in situ nick translation (ISNT). As a marker of proliferation, proliferating cell nuclear antigen (PCNA) was localized immunohistochemically. The numbers of nuclei positive with ISNT and for PCNA per 1,000 nuclei on tissue sections were obtained. The labelling indices were compared with clinicopathological characteristics for each tumour. The ISNT labelling index of well differentiated colon carcinomas was higher than that of poorly differentiated carcinomas. Among similarly differetiated cancers, ISNT L.I. of colon carcinomas classified as Dukes A was higher than Dukes B/C, and L.I. of carcinomas which did not metastasize to lymph node or liver was higher than that of carcinomas which metastasized. The PCNA labelling index did not correlate with any of the clinicopathological characteristics or with the ISNT labelling index. The data suggest that apoptosis indices severe as a marker of tumour progression.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s004280050095

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In situ localization of male germ cell-associated kinase (mak) mRNA in adult mouse testis: Specific expression in germ cells at stages around meiotic cell division (1992)

Koji, Takehiko ; Jinno, Atsushi ; Matsushime, Hitoshi ; [et al.]

New York, NY [u.a.] : Wiley-Blackwell

Cell Biochemistry and Function 10 (1992), S. 273-279

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ISSN: 0263-6484

Keywords: In situ hybridization ; v-ros tyrosine kinase ; male germ cell-associated kinase ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Medicine

Notes: Biochemical analysis of the male germ cell-associated kinase (mak) gene, which was isolated recently by using weak cross-hybridization with the v-ros tyrosine kinase gene, revealed that the gene was highly expressed in mammalian testicular germ cells, but not in ovarian cells. In order to identify the cells which express the mak gene in more detail, we localized mak mRNA in frozen sections of mouse testis by non-radioactive in situ hybridization. In this study, we utilized thymine-thymine (T-T) dimerized mak cDNA as a haptenic, non-radioactive probe, and the signal was detected enzyme-immunohistochemically by using an anti-T-T antibody. As a result, mak mRNA was localized intensely in late pachytene (stage X) and diplotene (stage XI) spermatocytes, and faintly in dividing spermatocytes (stage XII) and early round spermatids (stage I-II), suggesting that the gene may play an important role in the phase around meiotic cell division, but not throughout the entire meiosis.

Additional Material: 2 Ill.