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  • 1
    Electronic Resource
    Electronic Resource
    Cytological characterization of four meiotic mutants of Arabidopsis isolated from T-DNA-transformed lines (1997)
    Springer
    Chromosome research 5 (1997), S. 551-559 
    Details
    ISSN: 1573-6849
    Keywords: Arabidopsis ; fertility ; meiosis ; mutants ; T-DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A secondary screen of the Feldmann collection of T-DNA transformed Arabidopsis lines identified several meiotic mutants. We used a spreading technique combined with DAPI staining in a detailed cytogenetic analysis of meiotic chromosome behaviour in four of these mutants, all of which are putatively T-DNA tagged and therefore candidates for molecular and functional analysis of the mutated genes. Two of them are defined as ‘synaptic’ mutants, showing greatly reduced association of homologous chromosomes at metaphase I: one is asynaptic, showing failure of synapsis during prophase I, whereas the other is desynaptic and is characterized by normal but non-maintained synapsis. Another mutant is defective in meiotic cell cycle control and undergoes a third meiotic division, resembling a second division but without an additional round of chromosome duplication. A further mutant shows meiosis-limited chromosome disruption, resulting in extensive chromosome fragmentation combined with other defects. All four mutants experience very irregular chromosome distribution during the meiotic divisions, resulting in abnormal numbers and/or sizes of microspores, with resulting reduced fertility.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018497804129
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A light microscopic atlas of meiosis inArabidopsis thaliana (1996)
    Springer
    Chromosome research 4 (1996), S. 507-516 
    Details
    ISSN: 1573-6849
    Keywords: Arbidopsis ; cytogenetics ; light microscopy ; meiosis ; chromosomes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An atlas of meiosis inArabidopsis thaliana, encompassing all stages from preleptotene to telophase II and early microspore formation, is presented in detail for the first time. The photomicrographs and descriptions are based on staining with the DNA fluorochrome 4′,6-diamidino-2-phenylindole (DAPI) combined with a spreading procedure, or haematoxylin-iron alum (HIA) staining. Despite previous reservations about the practicality of cytogenetic meiotic analysis inArabidopsis due to its small genome size, good-quality and clearly analysable preparations of all meiotic stages were obtained. This atlas of normal, wild-type meiosis is considered an essential prerequisite to informed analyses of meiotic mutants. Furthermore, extended prophase I chromosomes, particularly at the pachytene stage, offer considerable potential for producing a detailed cytogenetic map (karyotype) ofArabidopsis chromosomes with the additional prospect of high-resolution physical mapping based on fluorescencein situ hybridization (FISH) of defined DNA probes to these extended chromosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02261778
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    Paper (German National Licenses)
    Fulltext
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