ISSN:
1573-0972
Keywords:
Bagasse
;
cellulase
;
β-glucosidase
;
lignocellulose
;
mixed cultures
;
solid-substrate fermentation
;
xylanase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract Ammonia-treated bagasse with 80%(w/w) moisture content was subjected to mixed-culture solid-substrate fermentation (SSF) with Trichoderma reesei LM-UC4 and Aspergillus phoenicis QM 329, in flask or pot fermenters, for cellulase production. Significantly higher activities of all the enzymes of the cellulase complex were achieved in 4 days of mixed-culture SSF than in single-culture (T. reesei) SSF. The highest filter-paper-cellulase and β-glucosidase activities seen in mixed-culture SSF were 18.7 and 38.6 IU/g dry wt, respectively, representing approx. 3- and 6-fold increases over the activities attained in single-culture SSF. The mixed-culture SSF process also converted about 46% of the cellulose and hemicellulose to reducing sugars and enriched the product with 13% fungal protein. The biomass productivity, 0.29 gl-1.h, and enzyme productivity, 28.0 IU I-1.h, were about twice as high in the mixed-culture than in the single-culture.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00367112
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