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  • 1
    Electronic Resource
    Electronic Resource
    Embryonic and post-natal changes in activity and molecular forms of mucosal cell butyrylcholinesterase in chicken intestine (1990)
    Springer
    Molecular and cellular biochemistry 96 (1990), S. 143-151 
    Details
    ISSN: 1573-4919
    Keywords: cholinesterase ; butyrylcholinesterase ; molecular forms ; mucosal cells ; chicken intestine ; development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary The mucosal cells of the chicken intestine contain a cholinesterase activity essentially due to butyrylcholinesterase. The enzyme is present during embryonic and post-hatching development. The activity reaches a maximum value at day 19 in ovo and decreases prior to and after hatching up to day 4 ex ovo. Then the activity again rises reaching a second maximum at 2–3 weeks. Beyond this stage, the activity slowly decreases leveling off to the value determined in adult chicken. The enzyme exists as two globular forms (G1 and G4) soluble at low-ionic strengths. The G4 form is predominant in ovo up to day 19. From this stage and after hatching the G1 form is the main one. This change in the form proportion differentiates the mucosal cell butyrylcholinesterase from butyrylcholinesterase of other origins such as the chicken plasma enzyme which always shows a predominant G4 form.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00420906
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization of cholinesterase molecular forms in the mucosal cells along the intestine of the chicken (1989)
    Springer
    Molecular and cellular biochemistry 85 (1989), S. 49-56 
    Details
    ISSN: 1573-4919
    Keywords: cholinesterase ; butyrylcholinesterase ; molecular forms ; mucosal cells ; chicken intestine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The presence of a butyrylcholinesterase (BuChE, EC 3.1.1.8) in the musocal cells of the chicken intestine was demonstrated by histochemical and biochemical methods. The study of its distribution, along the intestine from duodenum to rectum, showed that the jejuno-ileum possesses the highest activity. Sucrose gradient centrifugation revealed, in all intestinal areas, two globular forms with sedimentation coefficients of 4.3 S (G1 form) and 10.8 S (G4 form). The presence of Triton X-100 in the preparations did not modify the sedimentation profiles of these two forms which can be considered as soluble BuChE. The ratio of G1/G4-forms progressively decreases along the intestine from duodenum to rectum indicating a predominance of the G4 form in the areas where the activity is low. Our results are discussed in relation to other studies of globular forms of chicken BuChE.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223513
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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