ISSN:
1590-3478
Keywords:
cell culture
;
neurons
;
glial cells
;
chemically defined medium
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Description / Table of Contents:
Sommario Viene descritto un terreno chimicamente definito che facilita la sopravvivenza di neuroni di cervello di feto di ratto in coltura primaria. Più del 90% delle cellule in coltura vengono identificate come neuroni mediante immunofluorescenza con anticorpi antineurofilamenti. Il terreno, arricchito con siero, invece, stimolando la proliferazione delle cellule nonneuronali, ostacola la sopravvivenza dei neuroni.
Notes:
Abstract A Chemically Defined Medium is described which facilitates the survival of neurons in primary cultures of rat cerebral hemispheres. More than 90% of all cells were identified as neurons using neurofilament as a marker in an immunocytochemical assay. In contrast, serumsupplemented medium, by stimulating nonneuronal cell proliferation, hinders the survival of neurons in culture.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02043486
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