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  • RT-PCR  (1)
  • Receptor  (1)
  • nitrogen balance  (1)
  • 1
    ISSN: 1573-0867
    Schlagwort(e): Ammonia loss ; denitrification ; nitrogen balance ; algal growth ; transfer processes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Losses of nitrogen were investigated after applications of ammonium bicarbonate and urea to flooded rice at transplanting. Ammonia (NH3) volatilization was determined by direct micrometeorological methods, and total loss of fertilizer nitrogen (N) was measured by15N balance. All the loss appeared to be in gaseous forms, since there was no evidence of leaching and runoff was prevented. The difference between N loss and NH3 loss was thus assumed to be denitrification loss. Both NH3 volatilization and denitrification losses were large, being 39% and 33%, respectively, of the ammonium bicarbonate N, and 30% and 33%, respectively, of the urea N applied by farmers' methods. Ammonia fluxes from the field fertilized with ammonium bicarbonate were very high for two days, and then declined rapidly as the NH3 source in the floodwater diminished. Moderate fluxes from the field fertilized with urea continued over 6 days, but calculations showed that NH3 transfer from floodwater to atmosphere was retarded during the middle period of the experiment, particularly on day 2 when a thick algal scum appeared on the water surface. The results indicate that this algal mass obstructed the transport of NH3 across the water-air interface until the scum was dispersed by wind action. Nevertheless, the prolonged NH3 losses on the urea treatment were due primarily to high floodwater pH values promoted by the strong algal growth during the daylight hours. Nitrogen-15 balance studies showed that incorporation of fertilizer into drained soil substantially increased recoveries of fertilizer N in rice plants and soil compared with incorporation of fertilizer in the presence of standing floodwater. Ammonia loss measurements on these treatments when urea was applied suggested that the improvement in fertilizer N efficiency was due mainly to reductions in NH3 loss.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 42 (1995), S. 173-179 
    ISSN: 1040-452X
    Schlagwort(e): Embryonic stem cells ; Insulin ; IGF-I ; IGF-II ; Receptor ; RT-PCR ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie
    Notizen: Insulin and insulin-like growth factors (IGF-I and -II) are members of a family of growth factors which are known to be developmentally regulated during preimplantation mouse embryogenesis. The physiological actions of the insulin family of growth factors are mediated by interactions with specific cell surface receptors that are detectable on the cells of preimplantation mouse embryos. Mouse embryonic stem (ES) cells are totipotent cells derived directly from the inner cell mass of the blastocyst. ES cells have the ability to differentiate into all three germ layers and have unlimited growth potential under certain culture conditions. The great advantage of ES cells is the ability to obtain large amounts of tissue for biochemical studies as compared with preimplantation embryos. To examine in greater detail the biological actions of the insulin family of growth factors, the expression of their cognate receptors on ES cells was examined. ES cells were cultured in DMEM medium supplemented with leukemia inhibitory factor (LIF) to maintain the undifferentiated state. Receptor expression was evaluated at the mRNA level using the reverse transcription polymerase chain reaction (RT-PCR), and at the protein level by radioactive labeled ligand-receptor binding assay. Using RT-PCR, mRNAs of all three growth factor receptors were detected in ES cells. Messenger RNA from ES cells was reverse transcribed into cDNA by AMV reverse transcriptase at 42°C for 1 hr. The reverse transcription reaction was amplified with Taq polymerase and specific primers for insulin, IGF-I, or IGF-II receptors by PCR. RT-PCR and the control plasmid cDNA PCR products were resolved electrophoretically on 3% agarose gels. Each amplified PCR product showed the predicted correct size. The target sequence of RT-PCR amplified fragments were further verified by restriction enzyme digestion. The expression of receptors at the protein level was confirmed by Scatchard analysis, which showed specific binding of the radiolabeled ligands. This study shows that ES cells may provide a useful model to study the biological actions of the insulin family growth factors. © 1995 wiley-Liss, Inc.
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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