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Digitale Medien

Triiodothyronine receptor sites in serum-free cultured hepatocytes from adult rat liver (1985)

Goglia, F. ; Gallo, G. ; Palmero, S. ; [weitere]

New York, NY [u.a.] : Wiley-Blackwell

Cell Biochemistry and Function 3 (1985), S. 91-94

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Details

ISSN: 0263-6484

Schlagwort(e): Triiodothyronine ; nuclear T3 receptors ; hepatocyte primary cultures ; Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Medizin

Notizen: Nuclear T3 specific binding sites were characterized by Scatchard analyses of L-125I-T3 binding to nuclei extracted from freshly isolated and 1,2 and 6 day-cultured hepatocytes. The results demonstrate a marked decrease in T3 binding capacity of nuclei extracted from 1 day-cultured cells followed by an almost complete recovery within 6 days. The affinity constant value of nuclear receptor sites is significantly decreased in 1 day-cultured cells with a subsequent partial recovery. The affinity and capacity pattern of nuclear T3 binding sites appears to be in line with the delayed responses of hepatocyte primary cultures to T3.

Zusätzliches Material: 1 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/cbf.290030203

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