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  • 1
    ISSN: 1432-2048
    Schlagwort(e): Key words: Carbonic anhydrase ; Inorganic carbon ; Macroalga ; pH ; Photosynthesis ; Porphyra
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract. The capacity for HCO3 − use by Porphyra leucosticta Thur. in Le Jolis grown at different concentrations of inorganic carbon (Ci) was investigated. The use of HCO3 − at alkaline pH by P. leucosticta was␣demonstrated by comparing the O2 evolution rates measured with the O2 evolution rates theoretically supported by the CO2 spontaneously formed from HCO3 − . Both external and internal carbonic anhydrase (CA; EC 4.2.1.1) were implied in HCO3 − use during photosynthesis because O2 evolution rates and the increasing pH during photosynthesis were inhibited in the presence of azetazolamide and ethoxyzolamide (inhibitors for external and total CA respectively). Both external and internal CA were regulated by the Ci level at which the algae were grown. A high Ci level produced a reduction in total CA activity and a low Ci level produced an increase in total CA activity. In contrast, external CA was increased at low Ci although it was not affected at high Ci . Parallel to the reduction in total CA activity at high Ci is a reduction in the affinity for Ci, as estimated from photosynthesis versus Ci curves, was found. However, there was no evident relationship between external CA activity and the capacity for HCO3 − use because the presence of external CA became redundant when P. leucosticta was cultivated at high Ci. Our results suggest that the system for HCO3 − use in P. leucosticta is composed of different elements that can be activated or inactivated separately. Two complementary hypotheses are postulated: (i) internal CA is an absolute requirement for a functioning Ci-accumulation mechanism; (ii) there is a CO2 transporter that works in association with external CA.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    ISSN: 1573-5176
    Schlagwort(e): CO2 ; inorganic carbon ; macroalgae ; photosynthesis ; PAM
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Photosynthesis and cell composition of Porphyraleucosticta discs grown at low (〈 0.0001% in air), current (control) and high (1% CO2 in air)inorganic carbon (Ci) concentrations were analyzed. Carbohydrate content in discs grown at high Ci increased (15.1 mg g-1 FW) with respect to the control (6.4 mg g FW-1), whereas soluble protein content decreased to one-third (5.6 to2.1 mg g-1 FW). Carbohydrate content was unaffected and soluble protein slightly increased in discs grown at low Ci. As a consequence of these changes, a lower C/N molar ratio (8.6) was found in the discs grown at low compared to high Ci(12.4). Nitrate reductase activity increased at high Ci from 0.3 ± 0.2 to 1.7 ± 0.4 μmolNO2 - g-1 FW h-1indicating that reduction and assimilation of nitrate were uncoupled. The response of photosynthesis to increasing irradiance, estimated from O2evolution vs. irradiance curves, was affected by the treatments. Maximum quantum yield (Φ O2°) and effective quantum yield (Φ O2) at 150 μmol photon m-2s-1 decreased by 20% and 50%, respectively, at low Ci. These differences could be due to changes in photosynthetic electron flow between PSII and PSI. Treatments also produced changes in maximal (Fv/Fm) and effective (ΔF/Fm′)quantum yield for photosystem II charge separation.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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