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  • 1
    Electronic Resource
    Electronic Resource
    Large scale preparation of pure phycobiliproteins (1987)
    Springer
    Photosynthesis research 11 (1987), S. 225-235 
    Details
    ISSN: 1573-5079
    Keywords: phycobiliproteins ; phycocyanin ; allophycocyanin ; Microcystis aeruginosa ; cyanobacteria ; blue-green algae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This paper describes simple procedures for the purification of large amounts of phycocyanin and allophycocyanin from the cyanobacterium Microcystis aeruginosa. A homogeneous natural bloom of this organism provided hundreds of kilograms of cells. Large samples of cells were broken by freezing and thawing. Repeated extraction of the broken cells with distilled water released phycocyanin first, then allophycocyanin, and provides supporting evidence for the current models of phycobilisome structure. The very low ionic strength of the aqueous extracts allowed allophycocyanin release in a particulate form so that this protein could be easily concentrated by centrifugation. Other proteins in the extract were enriched and concentrated by large scale membrane filtration. The biliproteins were purified to homogeneity by chromatography on DEAE cellulose. Purity was established by HPLC and by N-terminal amino acid sequence analysis. The proteins were examined for stability at various pHs and exposures to visible light.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00055062
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Large scale preparations of photosynthetic catalysts from cyanobacteria (1996)
    Springer
    Photosynthesis research 47 (1996), S. 293-299 
    Details
    ISSN: 1573-5079
    Keywords: Arthrospira ; cytochromes ; ferredoxins ; flavoproteins ; phycobiliproteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Large scale preparations of photosynthetic catalysts from cyanobacteria afford special advantages in the discovery and the study of these proteins. Here, we present information on obtaining cyanobacteria from laboratory cultivation, commercial sources and from natural blooms. Procedures for the breakage and fractionation of large amounts of cells are reviewed. Since much of this information is not of a conventional sort, it may provide special help when the need for a large amount of a specific protein arises.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02184290
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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