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  • 1
    Digitale Medien
    Digitale Medien
    Fertile plant regeneration from protoplasts of meadow fescue (Festuca pratensis Huds.) (1993)
    Springer
    Plant cell reports 12 (1993), S. 95-100 
    Details
    ISSN: 1432-203X
    Schlagwort(e): forage grasses ; Festuca pratensis ; suspension cultures ; protoplasts ; plant regeneration
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Suspension cultures from mature embryo-derived compact callus were initiated in seven meadow fescue (Festuca pratensis Huds.) cultivars. Four to six months after initiation, embryogenic suspension cultures with a moderate growth rate were established from three of them (cvs. Barmondo, Belimo and Leopard). These suspension cultures showed the capacity, maintained over six months, to regenerate green plants which could be grown to maturity under greenhouse conditions. Morphogenic suspension cultures from single genotypes of three F. pratensis cultivars (cvs. Barmondo, Belimo and Leopard) yielded large numbers of protoplasts, which upon culture in agarose beads using nurse cells formed microcalli with an overall plating efficiency in the range of 10-3 to 10-4. Mature plants were reproducibly regenerated and established in soil, from such protoplasts during a period of six months. The regeneration of fertile plants from protoplasts derived from suspension cultures of meadow fescue and its implications on gene transfer technology for this species are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00241942
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Analysis of genetic stability of plants regenerated from suspension cultures and protoplasts of meadow fescue (Festuca pratensis Huds.) (1993)
    Springer
    Plant cell reports 12 (1993), S. 101-106 
    Details
    ISSN: 1432-203X
    Schlagwort(e): Festuca pratensis ; suspension cultures ; protoplasts ; plant regeneration ; somaclonal variation ; genetic fidelity
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A cytological and molecular analysis was performed to assess the genetic uniformity and true-to-type character of plants regenerated from 20 week-old embryogenic suspension cultures of meadow fescue (Festuca pratensis Huds.), and compared to protoplastderived plants obtained from the same cell suspension. Cytological variation was not observed in a representative sample of plants regenerated directly from the embryogenic suspensions and from protoplasts isolated therefrom. Similarly, no restriction fragment length polymorphisms (RFLPs) were detected in the mitochondrial, plastid and nuclear genomes in the plants analyzed. Randomly amplified polymorphic DNA markers (RAPDs) have been used to characterise molecularly a set of mature meadow fescue plants regenerated from these in vitro cultures. RAPD markers using 18 different short oligonucleotide primers of arbitrary nucleotide sequence in combination with polymerase chain reaction (PCR) allowed the detection of pre-existing polymorphisms in the donor genotypes, but failed to reveal newly generated variation in the protoplast-derived plants compared to their equivalent suspensionculture regenerated materials. The genetic stability of meadow fescue plants regenerated from suspension cultures and protoplasts isolated therefrom and its implications on gene transfer technology for this species are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00241943
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  • 3
    Digitale Medien
    Digitale Medien
    Experimental study of enhanced emission of the laser-ablated plume in backing gas (1994)
    Springer
    Applied physics 58 (1994), S. 143-148 
    Details
    ISSN: 1432-0649
    Schlagwort(e): 36.40
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract The effects of background gases on the optical emission of the excimer-laser-ablated plume from a brass target have been studied experimentally. It is found that the plume emission can be enhanced significantly in a proper gas ambient. In hydrogen, the highest peak intensity is detected, and in argon, there is a distinctive difference in the pressure-dependent emission between in He and in the other three gases, Ar, N2 and H2. Moreover, the monitored line peak intensity remains unchanged in Ar and N2 and increases in H2 within a distance above the target surface; but in He, the observed peak intensity decreases with distance like in vacuum. Furthermore, the emissions of several more atomic lines of Cu and Zn atoms from the plume are also found to be enhanced in the same manner in gas ambient. Some physical processes involved in the plume expansion and the possible mechanisms for the enhanced emission of the plume in backing gas are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01082349
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