ISSN:
1573-5044
Keywords:
Brassica alboglabra
;
Chinese kale
;
growth regulators
;
plant regeneration
;
tissue culture
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Culture conditions for shoot regeneration and proliferation, and rooting of Brassica alboglabra Bailey were optimized by a judicious selection of explants and manipulation of hormonal combinations in the culture medium. Both half and whole stem explants were more regenerative than cotyledons and hypocotyls. The highest shoot regeneration frequency (100%) accompanied by high number of shoots was obtained using half stem explants grown on Murashige & Skoog [14] medium supplemented with 2 mgl-1 BA in combination with 1 mgl-1 NAA, or 4 mgl-1 2iP with 0.5 mgl-1 NAA. For shoot proliferation, 4 mgl-1 kinetin was most effective. The presence of auxin reduced shoot proliferation significantly. Maximum rooting (100%) of shoot cuttings was obtained either in the absence or in the presence of 0.5 mgl-1 NAA, or IBA or IAA ranging from 0.1 to 8 mgl-1.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00046859
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