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Helix formation by poly{bis(hydroxyethyl)-L-glutamine} (1986)

Tilstra, L. ; Strickland, T. C. ; Clark, D. S. ; [et al.]

Springer

Colloid & polymer science 264 (1986), S. 469-472

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ISSN: 1435-1536

Keywords: Helix-coil transition ; poly(hydroxyalkyl-L-glutamine) ; poly{bis(hydroxyethyl)-L-glutamine}

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics

Notes: Abstract Circular dichroism spectra are reported for poly {bis(hydroxyethyl)-L-glutamine}. Its conformation is predominantly that of a statistical coil at or above ambient temperature in water. It becomes partially helical upon cooling. The helix-forming potential of this polypeptide in water is between that of poly(hydroxyethyl-L-glutamine) and poly(hydroxypropyl-L-glutamine). The polypeptide has a large helix content when the solvent is rich in trifluoroethanol.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01421998

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Transient responses of hybridoma cells in continuous culture to step changes in amino acid and vitamin concentrations (1994)

Hiller, G. W. ; Clark, D. S. ; Blanch, H. W.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 44 (1994), S. 303-321

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ISSN: 0006-3592

Keywords: hybridoma metabolism ; continuous culture ; suspension culture ; antibody productivity ; amino acids ; vitamins ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: The effects of step-change increase in the concentrations of amino acids and vitamins on the metabolism, growth, and antibody productivity of a murine hybridoma cell line grown in continuous culture on serum-free medium are presented. Additions of the amino acids cysteine with methionine, tryptophan, and isoleucine with valine and vitamin B12 (as cyanocobalamin) resulted in significant increases in viable cell concentrations. Additions of aspartate with asparagine, and threonine with vitamin B1 (as thiamine hydrochloride) resulted in significant increases in final antibody concentrations. Substantial decrease in the fraction of amino acid nitrogen excreted as ammonia occurred upon supplementation with three times the normal concentrations of branched chain amino acids. Decreases in the fraction of amino acid nitrogen converted to ammonia were paralleled by increases in the fraction converted to alanine. © 1994 John Wiley & Sons, Inc.

Additional Material: 11 Ill.