ISSN:
1432-0428
Keywords:
Key words Cytomegalovirus
;
Epstein
;
Barr virus
;
polymerase chain reaction
;
pancreas biopsy
;
autoimmunity
;
insulin-dependent diabetes mellitus.
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Viral infection is assumed to trigger or exacerbate autoimmune responses against pancreatic beta cells leading to the development of insulin-dependent diabetes mellitus (IDDM). We therefore examined by polymerase chain reaction the presence of two candidate viruses, cytomegalovirus and Epstein–Barr virus, in IDDM pancreases. Pancreas tissues were obtained by biopsy under laparoscopy from 16 recent-onset IDDM patients: age 17–53 years; disease duration 0–7 months; six had flu-like symptoms before onset. Frozen sections were made and subjected to DNA amplification. DNA samples were prepared from the frozen sections and polymerase chain reaction was performed using primers specific to cytomegalovirus, Epstein–Barr virus and control gene for HLA-DP. Cytomegalovirus- and Epstein–Barr virus-infected cells were used for positive control. Southern blot analysis could detect cytomegalovirus DNA from as few as 2 × 10–1 cytomegalovirus-infected cells and Epstein–Barr virus DNA from two Epstein–Barr virus-infected cells. This highly sensitive analysis, however, could not detect cytomegalovirus or Epstein–Barr virus genomes in pancreases of recent-onset IDDM. A single copy human gene (HLA-DP) was amplified from all IDDM pancreases indicating that DNA amplification was performed without inhibition. We conclude that cytomegalovirus or Epstein–Barr virus genomes are unlikely to exist in pancreas biopsy specimens of recent-onset IDDM patients. [Diabetologia (1995) 38: 667–671]
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00401837
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