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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 48 (1992), S. 272-277 
    ISSN: 1420-9071
    Keywords: Crustacean ; prawn ; hepatopancreas ; cell suspension ; protein synthesis ; α-amylase activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract In crustaceans, all the steps in the assimilation of food take place in the hepatopancreas. To facilitate the study of this organ, a method for the dissociation of cell types was developed. The hepatopancreas of the prawnPalaemon serratus was mechanically dissociated and the cells separated by Percoll density-gradient centrifugation. The E and R cells had similar densities of around 1.05 g/ml. The F cells were separated into two distinct fractions with densities of 1.075 and 1.082 g/ml. The B cells sedimented at a density of 1.12 g/ml. The ratio between the two populations of F cells was found to vary during the intermolt cycle while B cells disappeared after the molt. When the density gradient fractions were incubated with3H-leucine, incorporation was highest in the F cell fractions. Measurements of α-amylase activity, indicated that the two populations of F cells may be derived from the same cell type.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; N-Benzylideneanilines ; Hierarchical ascending classification ; Correspondence factor analysis ; Electronic effects ; Quantitative structure-retention relationships
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A series of 72 substitutedN-benzylideneanilines (NBA) has been studied by normal-phase liquid chromatography by use of an experimental design based on variation of the composition of mobile phases prepared from heptane and three modifiers, tetrahydrofuran, 1-octanol and ethyl acetate, for each of which the specific interactions are different. Seven mobile phases were defined in the experimental design. The chromatographic data obtained are used to discuss the behavior of NBA by application of complementary chemometric methods—hierarchical ascending classification (HAC) and correspondence factor analysis (CFA). Although solute polarity has the greatest effect on retention, construction of HAC and CFA plots shows that solute behavior is also influenced by second-order electronic effects arising as a result of specific interactions between the solutes and the different modifier solvents. A quantitative structure-retention relationship has been established between Hammett's constants and the solutes projection on the first factorial axis of CFA.
    Type of Medium: Electronic Resource
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