ISSN:
1573-5028
Keywords:
transformation
;
MgCl2-PEG-electroporation
;
competence
;
protoplasts
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Direct gene transfer into plant protoplasts has been recently developed, and conditions for high frequency transformation of SR1 tobacco protoplasts established. In this paper we analyse numerous transformation parameters in a comparative study on SR1 Nicotiana tabacum and N. plumbaginifolia, and report on a simple chemical technique for very efficient protoplast transformation. It is based on the synergistic interaction of MgCl2 and PEG. The technique yielded up to 1400 transformants per 3×105 treated N. tabacum protoplasts (up to 4.8% of the survivors, late selected clones). Using N. plumbaginifolia, the frequencies were 10-fold lower, indicating that the ‘competence’ for transformation has a species-specific component.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00015814
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