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  • 2000-2004  (2)
  • Muscle Calcium Troponin C  (1)
  • seeding effect  (1)
  • 1
    ISSN: 1432-2013
    Schlagwort(e): Muscle Calcium Troponin C
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract. Two genetically engineered, recombinant versions of native barnacle troponin C (TnC) (BTnC2) were created from the bacterially expressed, recombinant, wild-type BTnC (BTnCWT) to investigate the role of the Ca2+-specific sites in force regulation. The mutant BTnC4– contains a single amino acid mutation in site IV which results in the inactivation of site IV Ca2+ binding; the mutant BTnCTrunc lacks the last 11 amino acids of the C-terminal, and hence most of site IV. Both mutant proteins, which retain an active site II, bind to native TnC-depleted myofibrillar bundles and restore approximately 40% of the tension-generating capacity, about half that seen with purified native BTnC1 or BTnC2. This observation implies that the Mg2+-dependent interaction with troponin I (TnI) is at a location on TnC other than the C-terminal Ca2+-binding sites of BTnC2. Replacement with BTnCTrunc increases the sensitivity of the myofibrillar bundle to changes in ionic strength. Decreasing the ionic strength from 0.15 to 0.075 M increased force by 34%, a value much greater that the 8% increase seen in control bundles or bundles substituted with BTnC4–. These findings implicate TnC in determining this fibre characteristic, although this cannot be simply due to the alteration in the numbers of Ca2+ ions bound by the troponin complex since both BTnC4– and BTnCTrunc bind only 1 mol Ca2+/mol protein
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Journal of superconductivity 13 (2000), S. 575-581 
    ISSN: 1572-9605
    Schlagwort(e): texture YBCO ; BaZrO3 ; ultrafine particles ; pushing effect ; seeding effect
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Elektrotechnik, Elektronik, Nachrichtentechnik , Physik
    Notizen: Abstract We prepared precursors with fine BaZrO3 inclusions for melt-texture growth of high-temperature superconductors with nominal composition of Y1.5Ba2Cu3O y + x BaZrO3 (x = 0, 0.04, 0.08, and 0.1), by the process of liquid-phase powder melt-texture growth (LPMG). We then investigated the effects of the precursors on the texture growth and the microstructure of composite YBa2Cu3O7-δ/Y2BaCuO5 (YBCO). The BaZrO3 particles were about 100 nm in size in the textured YBa2Cu3O7-δ (Y123), observed by the scanning electron microscopy (SEM). Owing to the pushing effects of growing fronts, fine particles were pushed out of and thus gathered in front of the growing fronts or between Y123 grains. The gathered particles in turn blocked the further growth of the Y123 grains. However, BaZrO3 particles seeded Y123 grains, giving rise to multigrain growths. These negative effects of BaZrO3 fine particles exclude themselves as effective flux pinning centers in the textured YBCO.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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