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  • plastid RNA polymerase  (1)
  • single-tree selection method  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Plant molecular biology 36 (1998), S. 493-496 
    ISSN: 1573-5028
    Keywords: plastid promoter ; plastid RNA polymerase ; Hordeum vulgare
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transcription of plastid genes in higher plants is driven by two RNA polymerases. One is encoded in the chloroplast, the other is encoded in the nucleus. RNA synthesis in ribosome-deficient plastids is performed exclusively by the nuclear-encoded enzyme. In vitro capping was used to identify the transcriptional start sites of the genes clpP and rpl23 in ribosome-free plastids of the barley mutant albostrians. No transcript initiation was found at sequences similar to eubacterial promoters. Instead, transcription started near the motif 5′-YRTA-3′, which is also conserved in mitochondrial promoters of higher plants. Our data suggest that the nuclear encoded RNA polymerase is active in mature chloroplasts and is the sole polymerase involved in transcription of rpl123.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5095
    Keywords: natural regeneration ; seed-tree method ; single-tree selection method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The quantity and quality of seeds were studied in 886 shortleaf pine cones collected from 48 trees in each of two 15-ha natural stands in western Arkansas, USA. The number of seeds per cone ranged from 0 to 102, and averaged 34.6 and 38.0 in the two stands. Between 30 and 50 percent of the seeds were sound. Overall mean values for total number and total sound seeds compare with previous work. However, the wide variation between individual trees in a stand needs to be considered when evaluating cone abundance and estimating potential seed yields. For the stands observed in this study, a sample of 65 to 85 cones would be needed to estimate the number of sound seeds per cone (within ±3 seeds) with 95 percent probability. Sampling 1 or 2 cones from each of about 3 trees per ha would provide a reliable estimate of sound seed contents.
    Type of Medium: Electronic Resource
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