ISSN:
1573-6784
Keywords:
pulsed column reactor
;
enzymatic peptide synthesis
;
aqueous/organic biphasic medium
;
aspartame
;
thermolysin
Source:
Springer Online Journal Archives 1860-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract We propose a novel effective method for a continuous peptide synthesis in an aqueous/organic biphasic medium using a pulsed column reactor. N-Formyl-l-aspartyl-l-phenylalanine methyl ester was enzymatically synthesized continuously. With this extractive method using a pulsed column reactor, we can synthesize peptides with a stable performance even if a peptide (or a peptide-amino acid complex) is precipitated due to its high hydrophobicity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1008954129945
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