ISSN:
1432-1424
Keywords:
transcellular chloride transport
;
intracellular chloride activity
;
intracellular potassium activity
;
K+ chemical potential
;
Cl− chemical potential
;
chloride permeability
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary K+ and Cl−-selective double-barreled microelectrodes were used to study the effect of changes in external K+ concentration on intracellular Cl− activity (a Cl i ) in epithelial cells ofNecturus gallbladder. Decreasing the K+ concentration simultaneously in both bathing solutions produced a decrease ina Cl i . Steady-state values ofa Cl i were related to the values of the chamical potential gradient for K+ (ΔμK) across either the apical or the basolateral cell membrane. A similar dependence betweena Cl i and ΔμK appeared when the K+ concentration was changed in the serosal solution only. This indicates thata Cl i depends on ΔμK across the basolateral membrane.a Cl i was virtually independent of the membrane potential. This supports the idea that both the mucosal and the basolateral membranes ofNecturus gallbladder cells have very low passive permeabilities to Cl−. These results indicate that the exit of Cl− fromNecturus gallbladder cells is driven by ΔμK across the basolateral membrane, and suggest that a KCl electroneutral coupled mechanism in this membrane plays an important role in transcellular Cl− transport.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02000617
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