ISSN:
1573-9368
Keywords:
reporter genes
;
β-glucuronidase
;
transgenic plants
;
immunocytochemistry
;
in situ hybridization
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Among the methods now available to localize the sites of gene expression in plant materials, reporter genes based on thegus (uidA) gene ofEscherichia coli, which encodes a β-glucuronidase (E.C. 3.2.1.31; GUS), have been the most widely used during the last ten years. The apparent simplicity of the histochemical GUS assay has been a major factor in the increase in articles usinggus genes. However, over the last four years, there have been occasional reports expressing doubts concerning the specificity of the observed localizations based on discrepancies between results obtained with GUS histochemistry and immunocytochemistry and/orin situ hybridization. This brief review compares the results obtained with immunocytochemistry with those obtained with various GUS substrates for histochemical studies. Certain sources of artefact are discussed, as are the limits that should be imposed on interpretation of GUS histochemistry results at the organ, tissue and cell levels.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01968938
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