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  • 1
    Electronic Resource
    Electronic Resource
    Amylase and trypsin responses to intake of dietary carbohydrate and protein depend on the developmental stage in sea bass (Dicentrarchus labrax) larvae (1996)
    Springer
    Fish physiology and biochemistry 15 (1996), S. 237-242 
    Details
    ISSN: 1573-5168
    Keywords: amylase ; trypsin ; proteins ; carbohydrates ; marine fish larvae ; pancreatic maturation ; enzyme adaptation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sea bass (Dicentrarchus labrax) larvae were fed from day 15 to day 35 with 4 isoenergetic formulated diets that varied in protein (30, 40, 50, 60%) and carbohydrate (37, 27, 17, 7%) content. The diets were designated as P30, P40, P50 and P60, respectively. Best growth and survival were noted for P50 larvae. Poor growth and survival were observed for P30 larvae. The specific activity of amylase increased in direct relation to the dietary carbohydrate level from day 18 onwards. This increase was the result of extensive amylase synthesis. Trypsin activity was positively correlated with dietary protein level only at day 35. This study shows that the mechanisms involved in amylase regulation are efficient in very young sea bass larvae, while those related to trypsin appear later in ontogeny and thus suggests an age-dependent regulation of enzyme synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01875574
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Dietary regulation of activities and mRNA levels of trypsin and amylase in sea bass (Dicentrarchus labrax) larvae (1998)
    Springer
    Fish physiology and biochemistry 19 (1998), S. 145-152 
    Details
    ISSN: 1573-5168
    Keywords: sea bass ; larvae ; dietary adaptation ; gene regulation ; trypsin ; amylase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Specific activities and mRNA levels of trypsin and amylase were studied in sea bass larvae. From day 20 to day 40, Dicentrarchus labrax were fed two rations of one day old Artemia: satiation (LP) and one-eighth of the satiation ration (LP/8) or two isoenergetic compound diets that varied in protein (30 and 60%) and carbohydrate (37 and 7%) content (FP30 and FP60 respectively). Trypsin mRNA levels and specific activities were mainly influenced by the nature of dietary protein and the Artemia ration. By using fish meal as protein source, dietary protein concentration did not affect either mRNA level nor specific activity of trypsin. These results suggested that the trypsin synthesis was not affected at a transcriptional level by the protein ration, i.e., Artemia ration. Decrease in amylase mRNA observed from day 29 in the four dietary groups suggested that this decrease in amylase expression is genetically programmed during sea bass larvae development. Nevertheless, the composition and the quantity of the diet influenced the amylase specific activities revealing primarily translational regulation of amylase. This study shows for the first time that the molecular mechanisms which control the dietary adaptation of trypsin and amylase are independently regulated, age-dependent and influenced by the composition and the quantity of the diet.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007775501340
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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