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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 48 (1992), S. 367-371 
    ISSN: 1420-9071
    Keywords: Tunichrome ; phenylalanine ; ascidian ; tunicates ; blood pigments
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Ascidia ceratodes exposed to14C-phenylalanine in the surrounding seawater incorporates the radiolabel into newly biosynthesized tunichrome molecules. Radioactivity can be detected in tunichrome extracted from circulating blood cells within one day following initial exposure to the radiolabel; weak activity (≤4 μCi/mol tunichrome=22 nmol phenylalanine/mol tunichrome) is detected in 1 to 10 days; significantly higher amounts of radiolabel (57 μCi/mol tunichrome=318 nmol phenylalanine/mol tunichrome) appear 20 days after seawater exposure. Therefore, phenylalanine can function as a precursor in the biosynthesis of tunichrome.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1420-9071
    Keywords: Fluorescence activated cell sorting (FACS) ; tunicates ; morula cells ; signet ring cells ; vanadium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Specialized blood cells of many tunicates accumulate high concentrations of vanadium and phenolic peptide pigments called tunichromes (TC). In order to determine whether V and TC reside in the same cells,Ascidia nigra andAscidia ceratodes blood cell subpopulations were isolated by fluorescence-activated cell sorting (flow cytometry) and chemically analyzed. V was found in the spherical, gree/grey signet ring cells, and to a lesser degree in the mulberry-shaped, yellow/ green morula cells (MRs), whereas free TC was detected mainly in MRs.
    Type of Medium: Electronic Resource
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