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  • 1
    Electronic Resource
    Electronic Resource
    An insertion-deletion mutation in keratin 9 in three Chinese families with epidermolytic palmoplantar keratoderma (2005)
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 152 (2005), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2133.2005.06477.x
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  • 2
    Electronic Resource
    Electronic Resource
    A novel mutation of keratin 9 in a large Chinese family with epidermolytic palmoplantar keratoderma (2004)
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 150 (2004), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background  Epidermolytic palmoplantar keratoderma (EPPK) is an autosomal dominant inherited skin disorder characterized by diffuse yellow thickening of the skin of the palms and soles, sharply bordered with erythematous margins. Histologically and ultrastructurally, EPPK presents cytolysis of keratinocytes and abnormal aggregation of tonofilaments in the suprabasal layers of the epidermis. To date, 15 different mutations of the keratin 9 gene (KRT9) have been demonstrated to cause most cases of EPPK.Objectives  To identify the KRT9 mutation in a large Chinese family with EPPK.Methods  Denaturing high-performance liquid chromatography (DHPLC), DNA sequencing and allele-specific polymerase chain reaction (AS-PCR) were used to screen exon 1 of the KRT9 gene for sequence variations.Results  The DHPLC elution profiles of the DNA fragments amplified from the affected samples differed from those obtained from unaffected individuals, indicating that a sequence variation existed within the analysed fragment of KRT9. DNA sequencing revealed a novel insertion–deletion mutation in the exon 1 of KRT9, 497delAinsGGCT, resulting in the change of tyrosine166 to tryptophan and leucine (Y166delinsWL). AS-PCR confirmed the mutation was not a common polymorphism.Conclusions  The results suggest the molecular basis of EPPK in this Chinese family and provide further evidence that mutations in the helix initiation motif of keratin 9 underlie Chinese EPPK.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.0007-0963.2004.05865.x
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  • 3
    Electronic Resource
    Electronic Resource
    Modification of the reactivity of polyhydrides with Lewis acids. Synthesis and reactivity of {IrH5[P(iso-C3H7)3]2}2Cu(CF3SO3). X-ray crystal structure of {IrH2(MeCN)2[P(iso-C3H7)3]2}(BF4) (1990)
    s.l. : American Chemical Society
    Inorganic chemistry 29 (1990), S. 5000-5002 
    Details
    ISSN: 1520-510X
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ic00349a033
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  • 4
    Electronic Resource
    Electronic Resource
    Synthesis and characterization of the monomeric gallium monoamides tert-Bu2GaN(R)SiPh3 (R = tert-Bu, 1-adamantyl), Trip2MN(H)Dipp (M = Al, Ga; Trip = 2,4,6-iso-Pr3C6H2; Dipp = 2,6-iso-Pr2C6H3), and Trip2GaNPh2 (1993)
    s.l. : American Chemical Society
    Inorganic chemistry 32 (1993), S. 2557-2561 
    Details
    ISSN: 1520-510X
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ic00063a057
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  • 5
    Electronic Resource
    Electronic Resource
    Electrophysiological evidence for a spinal antinociceptive action of dipyrone (1994)
    Springer
    Inflammation research 41 (1994), S. 62-70 
    Details
    ISSN: 1420-908X
    Keywords: Dipyrone ; Metamizol ; Spinal cord ; Afferent fibres ; Inflammatory pain
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Electrophysiological experiments in anesthetized cats and rats were performed in order to study the effects of dipyrone on single afferent fibers from the knee joint and on spinal cord neurons with knee joint input. The neurons were activated and/or rendered hyperexcitable by an acute inflammation in the knee joint. In the joint nerve in cats, intravenous dipyrone (25–100 mg/kg) reduced ongoing activity in 10/12 thinly myelinated afferents but only in 1/10 unmyelinated afferents; the responses to movements of the inflamed knee were reduced in 8/10 thinly myelinated but only in 3/10 unmyelinated units. The reduction of activity was significant 20–30 min after application and was maximal at 60–180 min. In the spinal cord of spinalized cats, intravenous dipyrone (25–100 mg/kg) reduced ongoing activity and/or responses to pressure onto the inflamed knee in 14/16 neurons and in non-spinalized rats similar effects were seen in 10/11 neurons. Effects on spinal cord neurons started 5–10 min after application and were maximal after 20–40 min. These data show pronounced suppression of inflammation-induced nociception by dipyrone and they suggest that the spinal cord is a major site of action of this compound.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01986396
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  • 6
    Electronic Resource
    Electronic Resource
    On the Linear-Cost Subtree-Transfer Distance between Phylogenetic Trees (1999)
    Springer
    Algorithmica 25 (1999), S. 176-195 
    Details
    ISSN: 1432-0541
    Keywords: Key words. Evolutionary trees, Approximation algorithms, Lower bounds.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Mathematics
    Notes: Abstract. Different phylogenetic trees for the same group of species are often produced either by procedures that use diverse optimality criteria [16] or from different genes [12] in the study of molecular evolution. Comparing these trees to find their similarities and dissimilarities (i.e., distance ) is thus an important issue in computational molecular biology. Several distance metrics including the nearest neighbor interchange (nni) distance and the subtree-transfer distance have been proposed and extensively studied in the literature. This article considers a natural extension of the subtree-transfer distance, called the linear-cost subtree-transfer distance, and studies the complexity and efficient approximation algorithms for this distance as well as its relationship to the nni distance. The linear-cost subtree-transfer model seems more suitable than the (unit-cost) subtree-transfer model in some applications. The following is a list of our results: 1. The linear-cost subtree-transfer distance is in fact identical to the nni distance on unweighted phylogenies. 2. There is an algorithm to compute an optimal linear-cost subtree-transfer sequence between unweighted phylogenies in O(n ⋅ 2 O(d) ) time, where d denotes the linear-cost subtree-transfer distance. Such an algorithm is useful when d is small. 3. Computing the linear-cost subtree-transfer distance between two weighted phylogenetic trees is NP-hard, provided we allow multiple leaves of a tree to share the same label (i.e., the trees are not necessarily uniquely labeled). 4. There is an efficient approximation algorithm for computing the linear-cost subtree-transfer distance between weighted phylogenies with performance ratio 2 .
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00008273
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  • 7
    Electronic Resource
    Electronic Resource
    Parallel Algorithms for Maximal Acyclic Sets (1997)
    Springer
    Algorithmica 19 (1997), S. 354-368 
    Details
    ISSN: 1432-0541
    Keywords: Key words. Parallel algorithms, Maximal acyclic sets, Planar graphs.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Mathematics
    Notes: Abstract. Given a graph G=(V,E), the well-known spanning forest problem of G can be viewed as the problem of finding a maximal subset F of edges in G such that the subgraph induced by F is acyclic. Although this problem has well-known efficient NC algorithms, its vertex counterpart, the problem of finding a maximal subset U of vertices in G such that the subgraph induced by U is acyclic, has not been shown to be in NC (or even in RNC) and is not believed to be parallelizable in general. In this paper we present NC algorithms for solving the latter problem for two special cases. First, we show that, for a planar graph with n vertices, the problem can be solved in $O(\log^3 n)$ time with O(n) processors on an EREW PRAM. Second, we show that the problem is solvable in NC if the input graph G has only vertex-induced paths of length polylogarithmic in the number of vertices of G. As a consequence of this result, we show that certain natural extensions of the well-studied maximal independent set problem remain solvable in NC. Moreover, we show that, for a constant-degree graph with n vertices, the problem can be solved in $O(\sqrt{n}\log^3n)$ time with O(n 2 ) processors on an EREW PRAM.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00009178
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  • 8
    Electronic Resource
    Electronic Resource
    GD3 expression by cultured human tumor cells of neuroectodermal origin (1989)
    Springer
    Acta neuropathologica 79 (1989), S. 317-325 
    Details
    ISSN: 1432-0533
    Keywords: Neuroectodermal tumor cells ; Monoclonal antibody ; Ganglioside ; GD3
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Seven monoclonal antibodies (mAbs) reactive with ganglioside II3(NeuAc)2-LacCer (GD3) were generated; four of these mAbs (DMAb-21, DMAb-22, DMAb-23, and DMAb-24) by immunizing mice with GD3 adsorbed to Salmonella minnesota and the remaining three (DMAb-7, DMAb-8, and DMAb-17) with melanoma line SK-MEL 28, which contains 1.4 nmol sialic acid of GD3 per mg protein. The specificities of the mAbs were defined by high-performance thin-layer chromatography (HPTLC) immunostain and solid-phase radioimmunoassay (SP-RIA) with a panel of purified gangliosides. DMAb-7 and DMAb-8 reacted with GD3, IV3(NeuAc)2nLcOse4Cer(3′,8′-LD1), and very weakly with IV3(NeuAc)2II3NeuAc-GgOse4Cer (GTla), but not with II3NeuAc-LacCer (GM3), II3NeuAcGgOse3Cer(GM2), II3NeuAc-GgOse4Cer(GM1), II3NeuAc, IV3NeuAcGgOse4Cer (GD1a), II3(NeuAc)2GgOse3(GD2), II3(NeuAc)2GgOse4Cer (GD1b), IV3NeuAcII3(NeuAc)2, GgOse4Cer(GT1b), suggesting the binding epitope to be a terminal tetrasaccharide NeuAcα2-8NeuAcα2-3Galβ1-4(Glc or GlcNAc). DMAb-7 and DMAb-8 were used to investigate the expression of GD3 on cultured human tumor cells of neuroectodermal origin. Thirteen of 19 gliomas, 3 of 5 medulloblastomas, 5 of 5 neuroblastomas, 2 of 2 melanomas, and 1 of 3 teratomas were shown to react with DMAb-8 and/or DMAb-7 by cell surface-RIA (CS-RIA) and immunofluorescence (IF) assays. HPTLC and densitometric analysis confirmed these results, as positive immunostains in the GD3 region were obtained with oligoganglioside fractions from 9 glioma, 1 medulloblastoma, 2 neuroblastoma, 1 melanoma, and 1 teratoma cell line. Glioma cell line U-105 MG and medulloblastoma cell line Daoy contain GD3 as shown by HPTLC immunostain analysis of extracts, although GD3 was undetectable on the cell surface as determined by CS-RIA and IF. There was no detectable GD3 found in gangliosides isolated from cell lines U-373 MG, D-54 MG, TE-671, and PA-1, which were negative for both DMAb-7 and DMAb-8 by CS-RIA and IF assay. Our results provide evidence that GD3 is expressed extensively with significant quantitative heterogeneity on cultured human neuroectodermal tumor cells including glioma, medulloblastoma, neuroblastoma, and melanoma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00294668
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  • 9
    Electronic Resource
    Electronic Resource
    Disialoganglioside GD2 in human neuroectodermal tumor cell lines and gliomas (1991)
    Springer
    Acta neuropathologica 82 (1991), S. 45-54 
    Details
    ISSN: 1432-0533
    Keywords: GD2 ; Monoclonal antibodies ; Gliomas ; Ganglisides ; Medulloblastomas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Monoclonal antibodies (mAbs) recognizing the disialoganglioside II3(NeuAc)2GgOse3Cer (GD2) were produced by immunizing mice with the GD2-expressing neuroblastoma cell line LAN-1 and a prefusion boost with purified GD2 coupled to Salmonella minnesota. Two IgM mAbs were isolated which demonstrated high levels of reactivity (binding ratios in excess of 100) with GD2 by solid-phase radioimmunoassay and positivity in high-performance thin-layer chromatography (HPTLC) immunostain; only one (DMAb-20) was subsequently shown by analysis with a panel of defined ganglioside species to be specific for the minimum epitope of GD2, GalNAcβ1-4(NeuAcα2-8NeuAcα2-3)Gal-. DMAb-20 was used to evaluate the expression of GD2 by malignant glioma and medulloblastoma cell lines using cell surface radioimmunoassay, indirect membrane immunofluorescence, HPTLC immunostain, and densitometric analysis of extracted gangliosides from selected cell lines. Sixteen of 20 (80%) malignant glioma and 5 of 5 medulloblastoma cell lines reacted with DMAb-20; in agreement with previous studies, 5 of 5 neuroblastoma and 2 of 3 melanoma cell lines also reacted with DMAb-20. GD2 was proportionally increased in the glioma and medulloblastoma cell lines relative to levels in normal brain, as determined by densitometric analysis. In a phenotypic survey of malignant glioma biopsies, tumor cells in 24 of 30 (80%) cases stained positively with DMAb-20. Reactive astrocytes, both within and adjacent to tumors, were frequently intensely stained. Among the morphological variants of glioblastoma examined, the most intense staining with DMAb-20 was observed in neoplastic gemistocytes, with the weakest or absent staining in small cell glioblastomas. As GD2 is a commonly expressed surface antigen of gliomas and medulloblastomas, expression of which is retained in tissue culture, DMAb-20 will be useful in determining the functional role of GD2 in cell-cell interaction, adhesion, and invasion, and in defining altered growth control mechanisms of central nervous system neoplasms in in vitro models.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00310922
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  • 10
    Electronic Resource
    Electronic Resource
    Transformations of chemically fixed liquid anhydrous ammonia by soil microorganisms (1991)
    Springer
    Biology and fertility of soils 11 (1991), S. 145-150 
    Details
    ISSN: 1432-0789
    Keywords: Nitrogen-15 ; Ammonia fixation ; Organic matter-fixed NH3 ; Clay-fixed NH inf4 sup+
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The application of liquid anhydrous NH3 to soil leads to chemical fixation of NH3 by organic matter and of NH inf4 sup+ by clay minerals. A laboratory study was conducted to ascertain the biological transformations of newly fixed liquid anhydrous 15NH3 in a Drummer silty clay loam by incubation of the 15N-labelled soil with glucose for 0, 7, 30, and 90 days and by sequential extraction of organic-matter-fixed 15NH3 with 0.15 M Na4P2O7, 0.15 M KOH, 0.1 M NaOH, and acidified dimethyl sulfoxide. About 16% of the 15NH3 injected was fixed, of which 52% was accounted for by clay fixation. The various humic fractions (fulvic acid, humic acid, and humin) were obtained, and the distribution patterns of the fixed 15NH3-N in these fractions were compared. The potential availability of the fixed 15NH3-N was also estimated. The percentage of the 15NH3 recovered as organic-matter-fixed 15NH3 decreased as the length of incubation increased (to 28% after 90 days); the decrease was attributed in part to an increase in the amount recovered as clay-fixed NH inf4 sup+ (from 52 to 64%). Changes in the distribution of the organic-matter-fixed 15NH3-N in the humic fractions included: (1) an increase in the relative amount of the fixed 15NH3 as humic acid in both the Na4P2O7 and KOH extracts, (2) an increase in the percentage of organic-matter-fixed 15NH3-N in the fulvic acid fractions as high-molecular-weight components (determined by dialysis) or as generic fulvic acid (determined by sorption-desorption from XAD-8 resin), and (3) an increase in the percentage of the organic-matter-fixed 15NH3 as humin. The potential availability of the organic-matter-fixed 15NH3-N decreased as the length of the incubation increased, from 22 to 4% over the 90-day incubation period, and was correlated significantly (0.05 level) with Na4P2O7-extractable N. These results suggest that organic-matter-fixed liquid anhydrous NH3 is initially more labile than the native soil N but becomes less labile with time.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00336380
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