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  • 1
    Digitale Medien
    Digitale Medien
    A novel barley scald resistance gene: genetic mapping of the Rrs15 scald resistance gene derived from wild barley, Hordeum vulgare ssp. spontaneum (2005)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 124 (2005), S. 0 
    Details
    ISSN: 1439-0523
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Most genes for resistance to barley leaf scald map either to the Rrs1 locus on the long arm of chromosome 3H, or the Rrs2 locus on the short arm of chromosome 7H. Other loci containing scald resistance genes have previously been identified using lines derived from wild barley, Hordeum vulgare ssp. spontaneum. A single dominant gene conditioning resistance to scald was identified in a third backcross (BC3F3) line derived from an Israeli accession of wild barley. The resistance gene is linked to three microsatellite markers that map to the long arm of chromosome 7H; the closest of these loci, HVM49, maps 11.5 cM from the resistance gene. As no other scald resistance genes have been mapped to this chromosome arm, it is considered to be a novel scald resistance locus. As the Acp2 isozyme locus is linked to this scald resistance locus, at 17.7 cM, Acp2 is assigned to chromosome 7H. Molecular markers linked to the novel scald resistance gene, designated Rrs15, can be used in breeding for scald resistance.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1439-0523.2005.01085.x
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  • 2
    Digitale Medien
    Digitale Medien
    Linkage of Rust Resistance Genes from Wild Barley (Hordeum spontaneum) with Isozyme Markers (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 104 (1990), S. 0 
    Details
    ISSN: 1439-0523
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Eighty-three third backcross lines which comprise a set of near isogenic lines (NIL's) of the barley cultivar ‘Clipper’ but each carrying a different chromosomal segment from Hordeum spontaneum, marked with a distinct isozyme, were tested for resistance to three races of the barley leaf rust pathogen (Puccmia hordei). Fourteen lines showed resistance to at least one race and three showed resistance to all three races. The resistance in two of these lines was controlled by separate, single partially dominant genes. In one case the resistance gene named Rph1O was on chromosome 3 and linked (r = 0.15 ±0.05) with the isozyme locus Est2. In the second case, the gene (Rph11) was on barley chromosome 6 and linked (r = 0.07±0.02) with the isozyme locus Acp3 and (r = 0.11±0.02) with Dip2.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1439-0523.1990.tb00442.x
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  • 3
    Digitale Medien
    Digitale Medien
    Mapping of Malt Endopeptidase, NADH Diaphorase and Esterase Loci on Barley Chromosome 3L (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 112 (1994), S. 0 
    Details
    ISSN: 1439-0523
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Isoelectric focusing (IEF) and immunoblotting were used to detect genetic variants of malt endopeptidase (MEP1), an enzyme related to malting quality in barley and coded by the CepB locus on barley chromosome 3 (= 3H). A variant was found in a Turkish accession of wild barley (Hordeum vulgare ssp. spontaneum). The self progeny of a hybrid between this accession and the barley cultivar ‘Clipper’ were analyzed for recombination between the CepB locus and other isozyme loci. The estimates of recombination linked the CepB locus to an NADH diaphorase locus (Ndh2), which in turn was linked to the seedling esterase complex (Est1, Est2, and Est4) situated near the distal end of the long arm of chromosome 3. The Ndh2 locus was independent of two other NADH dehydrogenase loci (Ndh3, Ndh5) which were mapped on barley chromosome 5 (= 1H) in relation to the three hordein loci (Hor1, Hor2, and Hor3).
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1439-0523.1994.tb00685.x
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  • 4
    Digitale Medien
    Digitale Medien
    Genetic mapping of the barley Rrs14 scald resistance gene with RFLP, isozyme and seed storage protein markers (2000)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 119 (2000), S. 0 
    Details
    ISSN: 1439-0523
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: The scald susceptible barley cultivar ‘Clipper’ and a third-backcross (BC3) line homozygous for the Rrs14 scald resistance gene that originally came from Hordeum vulgare ssp. spontaneum were grown in replicated field trials. The level of resistance that Rrs14 confers against field populations of the pathogen Rhynchosporium secalis, the causal agent of scald disease, was evaluated. The Rrs14 BC3 line exhibited 80% and 88% less leaf damage than ‘Clipper’ in 1995 and 1996, respectively. Given this effectiveness of Rrs14, research was undertaken to identify a linked marker locus suitable for indirect selection of Rrs14. Based on linkage to a set of previously mapped loci, Rrs14 was positioned to barley chromosome 1H between the seed storage protein (hordein) loci Hor1 and Hor2, approximately 1.8 cM from the latter locus. The Hor2 locus is thus an ideal codominant molecular marker for Rrs14. The tight linkage between Rrs14 and Hor2 and the availability of alternative biochemical and molecular techniques for scoring Hor2 genotypes, permits simple indirect selection of Rrs14 in barley scald resistance breeding programmes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1046/j.1439-0523.2000.00456.x
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  • 5
    Digitale Medien
    Digitale Medien
    Variation in the Carbohydrate Composition of Wild Barley (Hordeum spontaneum) Grain (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 98 (1987), S. 0 
    Details
    ISSN: 1439-0523
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: The carbohydrate content of Hordeum spotaneum lines collected from 23 diverse environments in Israel was investigated. The range in composition was sucrose: 0.83—1.15%; trisaccharides): 0.48—1.16% total free and combined fructose: 1.2—4.2%; pentosans: 4.2—9.9%; and fl→3), (1→4)-β-glucans: 4.5—13.2%. The high pentosan and β-glucan content in some lines would cause quality problems if found in malting barleys.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1439-0523.1987.tb01100.x
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  • 6
    Digitale Medien
    Digitale Medien
    Use of Isozyme-Marked Segments from Wild Barley (Hordeum spontaneum) in Barley Breeding (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 100 (1988), S. 0 
    Details
    ISSN: 1439-0523
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Fifty-six accessions of Hordeum spontaneum from various Israeli habitats were hybridized with, and their progeny backcrossed three times to H. vulgare cv. ‘Clipper’. Selected chromosome segments marked by one of 24 isozyme loci were transferred to form 84 backcross lines which were nearly isogenic with ‘Clipper’. Each line was made homozygous for a single isozyme-marked segment. These lines were evaluated in preliminary and advanced field trials. The yield, malt extract percentage and grain size of the lines approached or equalled, and sometimes significantly surpassed, that of ‘Clipper’ and more recent commercial cultivars. Three isozyme-marked segments were combined in pairs and the three resultant doubly-marked lines were compared with the reconstituted parental, singly-marked lines and with the ‘Clipper’ recombinant from the same F2-array. The additive effects of single segments on yield or gram size were not significant, whereas half of the six interactions were significant, but in the unfavorable direction. It was concluded that near-isogenic lines can identify segments of wild barley germplasm that are useful for improving yield, but that pairs of these from different habitats may interact unfavourably.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1439-0523.1988.tb00254.x
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  • 7
    Digitale Medien
    Digitale Medien
    The charge-state model of protein polymorphism in natural populations (1975)
    Springer
    Journal of molecular evolution 6 (1975), S. 149-163 
    Details
    ISSN: 1432-1432
    Schlagwort(e): Electrophoretic Detectability ; Neutral Mutation Theory ; Evolution ; Mutation Rates
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Routine electrophoretic surveys for genetic variation in natural populations depend primarily upon detecting differences in the net charge carried by a protein. We have calculated the proportion of base substitutions which would yield an electrophoretically detectable mutant protein, and the relative mutation rates among different charge classes, under a variety of simplifying assumptions. These calculations indicate that: (i) only 25 per cent of all single base mutations would lead to a charge change on a protein molecule. (ii) five distinct classes of electrophoretic variants can be generated from a specified protein by single base substitutions. (iii) the relative mutation rates differ markedly among the different charge classes which can be generated by single base substitutions. The estimates of the proportion of electrophoretically detectable mutant proteins and relative mutation rates among charge classes were relatively robust to changes in assumptions concerned with the kind and site of base substitutions and the amino acid composition of the protein.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01732353
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  • 8
    Digitale Medien
    Digitale Medien
    Genetic diversity in the wild progenitor of barley in Israel (1979)
    Springer
    Cellular and molecular life sciences 35 (1979), S. 1027-1029 
    Details
    ISSN: 1420-9071
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Genetic structure of populations ofHordeum spontaneum, the wild progenitor of barley, was studied electropho-retically in proteins encoded by 28 gene loci in 1179 individuals representing 28 populations covering the entire ecological range in Israel; for comparison, the same test was conducted in 100 random seeds of Composite Cross XXI (generation 17) of cultivated barley,Hordeum vulgare. The results indicate that: a)H. spontaneum in Israel is genetically highly polymorphic, b) clinal, regional and local genetic patterns are significantly correlated with and predictable by climatic and soil variables, suggesting the operation of natural selection, and c) natural populations are on the average more variable than the tested Composite Cross generation. This genetic variation awaits testing and exploitation in breeding programs.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01949920
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  • 9
    Digitale Medien
    Digitale Medien
    Biochemical genetics of some seed proteins of Pinus radiata (1981)
    Springer
    Biochemical genetics 19 (1981), S. 585-597 
    Details
    ISSN: 1573-4927
    Schlagwort(e): seed proteins ; Pinus radiata ; size polymorphism
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract In a high-salt soluble fraction of the total protein from single seeds of Pinus radiata, up to 45 polypeptides were resolved on SDS-polyacrylamide gels. At least one-fifth of these polypeptides showed variation between seeds. In the 27,000–29,000 dalton region, two polypeptides were inherited as codominant alleles at a single locus and were shown to assort independently of another seed protein locus and three allozyme loci. A survey of 120 individuals from the five known native populations of P. radiata in California detected only the 27K and 29K alleles at the locus. In all populations, the 29K allele predominated, and the two island populations were monomorphic for the 29K allele. The 27 and 29 kdalton polypeptides were shown to have very similar amino acid sequences, and the allelic difference at this locus is most probably in the gene sequence for the polypeptide.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00484628
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  • 10
    Digitale Medien
    Digitale Medien
    Three alcohol dehydrogenase genes in wild and cultivated barley: Characterization of the products of variant alleles (1984)
    Springer
    Biochemical genetics 22 (1984), S. 495-515 
    Details
    ISSN: 1573-4927
    Schlagwort(e): alcohol dehydrogenase ; isozymes ; barley ; anaerobic induction
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Barley (Hordeum vulgare) and its wild progenitor (H. spontaneum) have three loci for alcohol dehydrogenase (EC 1.1.1.1; ADH). The Adh1 locus is constitutively expressed in seed tissues, whereas expression of the loci Adh2 and Adh3 requires anaerobic induction. The Adh3 gene is well expressed in aleurone and embryo tissues kept under N2 for 2–3 days. Using N2-treated embryos, a diverse collection of H. spontaneum was screened in starch gels for electrophoretic variants at the Adh3 locus. Four variants were found: two were conventional mobility variants (Adh3 S, Adh3 V); one was a null variant (Adh3 n); and the fourth (Adh3 I) variant lacked active homodimers and showed reduced heterodimer activity. The 35S-labeled monomers induced under N2 in the lines homozygous for Adh1, Adh2, or Adh3 variants were immunoprecipitated with antiserum raised against maize ADH. Fluorography after separation by SDS-PAGE and by urea-isoelectric focusing indicated that the Adh3 n allele was CRM- and that the Adh3 I gene product was smaller than normal. The Adh1 and Adh3 variants showed independent segregation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00484519
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