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Digitale Medien

System Peaks of Cyclodextrins in Capillary Electrophoresis (2000)

Chen, Yet Ran ; Ju, Dar Der ; Her, Guor Rong

Weinheim : Wiley-Blackwell

Journal of High Resolution Chromatography 23 (2000), S. 409-412

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Details

ISSN: 0935-6304

Schlagwort(e): Cyclodextrin ; capillary electrophoresis ; system peak ; CHES 1 ; Chemistry ; Analytical Chemistry and Spectroscopy

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie

Notizen: Using a running buffer containing cyclodextrins (CDs) and 2-[N-cyclohexylamino]-ethanesulfonic acid (CHES), positive system peaks were observed in the analysis of a ganglioside mixture by CE-UV. These system peaks were related to CDs in the running buffer because these peaks were also detected when a plug of solution devoid of CDs but having the same CHES concentration and pH as the running buffer was injected. Neutral CDs were separated owing to the formation of inclusion complexes with the anionic CHES ion. One possible explanation for the positive system peaks is that the anionic CD-CHES inclusion complex is displaced by co-ions with higher UV absorptivity.

Zusätzliches Material: 3 Ill.

Materialart: Digitale Medien

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