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  • 1
    Digitale Medien
    Digitale Medien
    Cartilage proteoglycan degradation by a mouse transformed macrophage cell line is mediated by macrophage metalloelastase (1999)
    Springer
    Inflammation research 48 (1999), S. 280-288 
    Details
    ISSN: 1420-908X
    Schlagwort(e): Key words: Macrophage metalloelastase — Activation — Cartilage degradation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract. Objective and Design: Identify and characterize the matrix metalloproteinase responsible for cartilage proteoglycan degradation mediated by a macrophage cell line in a cell culture model that resembles some aspects of rheumatoid pannus.¶Materials or Subjects: Supernatants from the transformed mouse macrophage cell line J774A.1 were used to purify the proteoglycan degrading activity.¶Methods: J774A.1 macrophage culture supernatants were purified by sequential column chromatography and proteins were identified by zymography, western blotting and amino acid sequence analysis. Cartilage degradation was measured using 35S labeled bovine nasal cartilage.¶Results: The cartilage degrading proteolytic activity in the mouse macrophage supernatants proved to be due to two major proteins with approximate molecular masses of 48 kDa and 22 kDa that were identified as macrophage metalloelastase (MME). Incubation of purified MME at 37°C for up to 16 h resulted in the processing of the 48 kDa protein to several novel bands including a previously undescribed protein of ∼25 kDa without accumulation of fully processed 22 kDa protein. A number of proteinases increased the rate of this processing. J774A.1 macrophage metalloelastase degraded cartilage proteoglycan with an efficiency approximately equal to human macrophage metalloelastase (MMP-12) and matrilysin (MMP-7) and twice that of stromelysin-1 (MMP-3).¶Conclusions: These data identify the cartilage proteoglycan degrading metalloproteinase secreted by J774A.1 macrophages in this cell culture model as MME, and describes mechanisms of activation and processing of this enzyme that may play an important role in cartilage degradation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s000110050460
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  • 2
    Digitale Medien
    Digitale Medien
    Human chondrosarcoma cells stably transfected with a stromelysin (MMP-3) promoter reporter construct provide a method of assessing transcriptional inhibitors (1997)
    Springer
    Inflammation research 46 (1997), S. 159-160 
    Details
    ISSN: 1420-908X
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s000110050157
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  • 3
    Digitale Medien
    Digitale Medien
    Inhibition of cartilage degradation in rat collagen-induced arthritis but not adjuvant arthritis by the neutrophil elastase inhibitor MDL 101,146 (1997)
    Springer
    Inflammation research 46 (1997), S. 503-508 
    Details
    ISSN: 1420-908X
    Schlagwort(e): Key words: Neutrophil elastase — Adjuvant arthritis — Collagen arthritis — MDL 101,146 — Cartilage degradation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract. Objective and Design: The neutrophil elastase inhibitor MDL 101,146 was examined for its anti-arthritic effect and to determine the role of neutrophil elastase in collagen-induced arthritis and adjuvant arthritis.¶Material: The collagen-induced arthritis model was performed in female DA rats immunized on day 0 and 7 with chick type II collagen in Freund's incomplete adjuvant. Adjuvant arthritis was induced in female Lewis rats by an intradermal injection of heat-killed Mycobacterium tuberculosis H37RA.¶Methods: The clinical signs of arthritis were assessed, joint swelling was measured using calipers and bone degradation, new bone proliferation, pannus formation and cartilage degradation were evaluated histologically.¶Results: MDL 101,146 administered orally inhibited the severity of collagen-induced arthritis as measured by a reduction in clinical score and joint swelling. Histological evaluation demonstrated a bone and cartilage sparing effect of MDL 101,146 in the tibio-tarsal joint of animals with collagen-induced arthritis.¶Conclusions: These results suggest that destruction of the joint in collagen-induced arthritis is at least partially due to neutrophil elastase.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s000110050233
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