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  • 1
    Digitale Medien
    Digitale Medien
    Developmental changes of sugar occurrence and distribution in the rat submandibular and sublingual glands (1999)
    Springer
    Anatomy and embryology 199 (1999), S. 113-123 
    Details
    ISSN: 1432-0568
    Schlagwort(e): Key words Morphogenesis ; Histochemistry ; Lectins ; Carbohydrates ; Salivary glands ; Rat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract  The developmental expression of salivary glycoconjugates was investigated in the rat submandibular and sublingual glands by conventional and lectin histochemistry. By the time of the first differentiation of secretory structures, in spite of similar morphological features, a different histochemical reactivity was detected, accounting for a relevant content of neutral glycoconjugates in the submandibular gland and the occurrence of both neutral and acidic glycoconjugates in the sublingual one. The use of lectins allowed the main changes of secretory components to be noted around gestational day 18. DBA and WGA lectins seemed to act as pre- and post-natal development markers while Con A lectin was indicative of post-natal differentiation. Taken together, data from lectin histochemistry indicated the transitional occurrence of glycoconjugates, probably involved in temporally restricted functions, as well as the co-existence of different secretory components that might also reflect maturational changes of single products.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004290050214
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  • 2
    Digitale Medien
    Digitale Medien
    Further cytochemical studies of the mucopolysaccharides of the test cells of Ciona intestinalis L. (1969)
    Springer
    Histochemistry and cell biology 19 (1969), S. 58-63 
    Details
    ISSN: 1432-119X
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary A morphological and cytochemical study (mucopolysaccharides, proteins and DNA) was made of the test cells of oocytes of Ciona intestinalis collected in February, April, July and September. The tests revealed an increase in the number of PAS-positive test cells in oocytes of 60–80 μ and of 90–100 μ during February and April and a parallel increase during these periods of mitotic activity in the cells in question. The findings obtained in this study, a continuation of earlier investigations, are discussed in the light of the possible links that might be established between the vic-glycol groups of the neutral mucopolysaccharides and the proteins.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00305962
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  • 3
    Digitale Medien
    Digitale Medien
    Distribution of lectin binding sites in rabbit oviduct (1985)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 211 (1985), S. 279-284 
    Details
    ISSN: 0003-276X
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin
    Notizen: The carbohydrate histochemistry of the rabbit oviduct has been examined by the use of four lectins conjugated with horseradish peroxidase as histochemical reagents. Each lectin gave a very distinct typical pattern of binding, but for each lectin there was no difference between the distribution of binding sites in ampulla and isthmus. Wheat germ lectin bound exclusively with the connective tissue of the oviduct folds; winged pea lectin was detected only in the ciliated cells; peanut lectin binding sites were visualized in the secretory cells; the binding reactivity of soybean lectin was limited to the basal part of the cilia. Although it is very difficult at present to correlate the distribution of lectin binding sites with the function of the positive cells, some hypotheses have been advanced.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/ar.1092110308
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  • 4
    Digitale Medien
    Digitale Medien
    Lectin binding and identification of sialic acid acceptor sugars in rabbit oviduct under hormone administration (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Microscopy Research and Technique 31 (1995), S. 488-496 
    Details
    ISSN: 1059-910X
    Schlagwort(e): Lectins ; Ampulla ; Isthmus ; Rabbit ; HCG ; Estradiol ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Allgemeine Naturwissenschaft
    Notizen: Localization of individual glycosidic residues and sialic acid acceptor sugars was investigated by conjugated lectins in the rabbit oviduct under physiological hormonal conditions and human chorionic gonadotropin (HCG) administration. Ampulla and isthmus were found to exhibit lectin binding profiles typical of each hormonal stage. Two different sialylated glycomolecules were identified within the epithelial lining; in particular, sialoglycoconjugates characterized by the terminal sequence sialic acid-galactose were visualized in the secretory cells and the sialic acid-N-acetylgalactosamine terminal disaccharides were localized on both ciliated and secretory cells of the entire oviduct. Surface and cytoplasmic sialoglycoconjugates were also found to exhibit a differential behaviour inside the two oviduct tracts examined. Present findings further supported the idea that in ampulla and isthmus, the greatest modifications consequent to hormone treatment take place at different times. © 1995 Wiley-Liss, Inc.
    Zusätzliches Material: 18 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/jemt.1070310605
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