ISSN:
1471-4159
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Medizin
Notizen:
The insecticide DDT (1,1,1 trichloro-2,2-bis-(p-chlorophenyl) ethane) was found to affect the amount of 32P from [γ-32P] ATP incorporated into proteins derived from lobster peripheral nerves in a variety of ways depending upon the relative concentrations of ATP, Na+, K+, Mg2+, and Ca2+. When a high concentration of ATP (2.5 ± 10−5m) was used DDT (10−5m) inhibited the incorporation and reversed the increased incorporation caused by ouabain. At low concentration of ATP (8.6 ± 10 −8m), DDT inhibited the incorporation when the buffer contained Mg2+ and Na+ or K+ alone, but when the buffer contained Mg2+ and both Na+ and K+ together, DDT consistently caused an increased amount of 32P to be incorporated. The ability of DDT to cause increases in incorporation of 32P into the lobster nerve proteins was found to parallel many of the properties that this compound shows when allowed to poison crustacean nerves. For example: the effect was negatively temperature dependent, Ca2+ lowered the incorporation in the presence of DDT, and DDT consistently caused greater amounts of 32P to be incorporated than its less potent analog DDE (1,1-dichloro-2,2-bis-(p-chlorophenyl)ethylene). The proteins that were affected by DDT were microsomal in nature and could be centrifuged from supernatant by recentrifuging at 149,000 g. The possibility that this system may be the actual target through which DDT causes its characteristic interferences of the ionic conductance changes associated with the action potential are discussed.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1111/j.1471-4159.1974.tb04292.x
Permalink