ISSN:
1744-313X
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Biologie
,
Medizin
Notizen:
The blood from three individuals belonging to consecutive generations of one family were characterized as cis AB on the basis of (a) the serological behaviour of the B antigen on their red cells, (b) the presence of weak anti-B in their sera and (c) family studies, which unequivocally demonstrated the genotype AB: O for two of the individuals with atypical AB groups.The serological behaviour of the A antigen on the red cells was intermediated between that of normal A1 and A2 cell. The B antigen gave weak and variable reactions with naturally occurring antibodies. Treatment of the cis AB red cells with an α-galactosyltransferase in the serum from an A1B individual rendered them agglutinable by the normal range of anti-B sera, demonstrating that the red cells do not lack precursors for the formation of normal B-active structures.The cis AB sera all contained relatively strong α-N-acetylgalactosaminyl-transferases which had pH optima of 6.0, characteristic of A1 gene-specified enzymes. The affinity of the enzymes for UDP-galactose, as measured by the apparent Ki, appeared to be greater than that of the A transferases in normal A1 and A1B sera. The B-gene transferases in the cis AB sera were very weak and were more readily inhibited by UDP-N-acetylgalactosamine than were the α-galactosyltransferases in normal B and AB sera. Both the A- and B-transferase activities in the cis AB sera are, therefore, in some ways different from the corresponding enzyme activities in the sera of normal A, B or AB donors.Cytogenetic study of blood lymphocytes from all three individuals showed normal karyotypes. Specifically, the terminal bands of the long arms of the No. 9 chromosomes, to which the ABO:Np-1: AK-1 linkage group has been assigned, displayed G bands similar to those in cells from other (normal) individuals examined simultaneously.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1111/j.1744-313X.1978.tb00634.x
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