ZIB

Treffer pro Seite

Treffer 1 - 3 | 3 Treffer

Sortierung

Digitale Medien

Enzyme reaction rates determined by fast atom bombardment mass spectrometry (1984)

Smith, Laurence A. ; Caprioli, Richard M.

Chichester : Wiley-Blackwell

Biological Mass Spectrometry 11 (1984), S. 392-395

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 0306-042X

Schlagwort(e): Chemistry ; Analytical Chemistry and Spectroscopy

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie

Notizen: Fast atom bombardment (FAB) mass spectrometry has been used to measure the reaction rate of trypsin with the synthetic substrate p-toluenesulfonyl-L-arginine methyl ester (TAME). Rates were measured by FAB mass spectrometry both in real time by monitoring the reaction as it proceeded on the probe inside the mass spectrometer, and also by individual analysis of a series of aliquots removed with time from a batch reaction. The results were then compared to the rates measured using the usual spectrophotometric (UV) assay run with the same reaction mixture. The results showed that the initial rates of trypsin cleavage were the same for these three methods at approximately 0.15 μmol product produced min-1 μg-1 enzyme. After 5 min, the rates determined by FAB mass spectrometry using timed aliquots and the UV assay remained about the same, whereas the real-time measurement decreased substantially to approximately 0.002 μmol product min-1 μg-1 enzyme. The results are discussed with respect to the particular advantages and disadvantages of these methods for following enzyme reactions.

Zusätzliches Material: 5 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/bms.1200110805

Permalink

Bibliothek	Standort	Signatur	Band/Heft/Jahr	Verfügbarkeit

Andere fanden auch interessant ...

Artikel (Nationallizenzen)

Volltext

Digitale Medien

Performance of a fast atom bombardment source on a quadrupole mass spectrometer (1983)

Caprioli, Richard M. ; Beckner, Carl F. ; Smith, Laurence A.

Chichester : Wiley-Blackwell

Biological Mass Spectrometry 10 (1983), S. 94-97

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 0306-042X

Schlagwort(e): Chemistry ; Analytical Chemistry and Spectroscopy

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie

Notizen: A saddle field fast atom bombardment gun was installed on a quadrupole mass spectrometer and its performance evaluated. Only some minor modifications to a standard gas chromatography mass spectrometry system were required to accommodate this source. Using xenon as the bombarding atom, measurements were made with respect to sensitivity, background effects, sample type, calibration of the data system and other parameters pertinent to the operation of a fast atom bombardment source on a quadrupole system. The simplicity of operation and its unique ability to produce gaseous ionic species from highly polar molecules in solution make it an ideal complement to other mass spectrometric ionizing devices.

Zusätzliches Material: 4 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/bms.1200100209

Permalink

Bibliothek	Standort	Signatur	Band/Heft/Jahr	Verfügbarkeit

Andere fanden auch interessant ...

Artikel (Nationallizenzen)

Volltext

Digitale Medien

Following enzyme catalysis in real-time inside a fast atom bombardment mass spectrometer (1983)

Smith, Laurence A. ; Caprioli, Richard M.

Chichester : Wiley-Blackwell

Biological Mass Spectrometry 10 (1983), S. 98-102

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 0306-042X

Schlagwort(e): Chemistry ; Analytical Chemistry and Spectroscopy

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie

Notizen: Experiments involving the monitoring of enzyme catalyzed reactions occurring within a fast atom bombardment mass spectrometer are reported. Data are presented using FAB mass spectrometry to monitor trypsin and dipeptidyl aminopeptidase I catalyzed hydrolysis of their respective assay substrates and for hydrolysis of the peptide (val4) angiotensin III by yeast carboxypeptidase Y. Enzyme catalysis was followed by measuring the decrease of abundance of the [M + H]+ ion of the substrate and the increase of that of the product from a mixture of enzyme and substrate in a glycerol/water matrix placed on the probe inside the mass spectrometer. In addition, results of preliminary experiments to determine the survival of live yeast cells on exposure to FAB mass spectrometry is also presented.

Zusätzliches Material: 5 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/bms.1200100210

Permalink

Bibliothek	Standort	Signatur	Band/Heft/Jahr	Verfügbarkeit

Andere fanden auch interessant ...

Artikel (Nationallizenzen)

Volltext

Treffer 1 - 3 | 3 Treffer