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Digitale Medien

Mapping studies and genetic analysis of transfer genes of the multi-resistant IncHI2 plasmid, R478 (1999)

Page, David T ; Whelan, Kenneth F ; Colleran, Emer

Oxford, UK : Blackwell Publishing Ltd

FEMS microbiology letters 179 (1999), S. 0

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ISSN: 1574-6968

Quelle: Blackwell Publishing Journal Backfiles 1879-2005

Thema: Biologie

Notizen: A bank of transfer-defective Tn7 insertion mutants of the multi-resistant IncHI2 megaplasmid, R478, was generated. Complementation analysis of these mutations identified a large 144-kb transfer-associated region of R478. A 6.8-kb segment from the transfer region was sequenced. The precise locations of Tn7 insertion within four distinct R478::Tn7 transfer-defective mutants were mapped and each insertion was found to disrupt a specific open reading frame. These transfer-associated determinants of R478 were designated htdB (H transfer determinant), htdD, htdT and htdC. Both htdB and htdC encoded amino acid sequences that showed a low homology with pilus biosynthetic proteins encoded by the F plasmid.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1111/j.1574-6968.1999.tb08702.x

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