ZIB

1

Digitale Medien

Cloning, nucleotide sequence and expression of the gene encoding the cellulose-binding protein 1 (CBP1) of Fibrobacter succinogenes S85 (1996)

Mitsumori, Makoto ; Minato, Hajime ; Sekizaki, Tsutomu ; [weitere]

Oxford, UK : Blackwell Publishing Ltd

FEMS microbiology letters 139 (1996), S. 0

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 1574-6968

Quelle: Blackwell Publishing Journal Backfiles 1879-2005

Thema: Biologie

Notizen: Abstract The nucleotide sequence of the gene encoding the Fibrobacter succinogenes S85 cellulose-binding protein 1 (CBP1) has been determined. The gene encodes a protein of 1054 amino acids with a molecular mass of 118614. The deduced amino acid sequence of CBPl showed an extensive similarity to the cellulose-binding domain of an endoglucanase (EGCCD) from Clostridium cellulolyticum and contained the reiterated regions. The cloned gene was inserted into an expression vector, pRSETA, and was expressed in E. coli as a fused protein with the peptide consisting of six consecutive histidine residues. The fused protein was detected by immunoblotting using antiserum against CBP1, and exhibited the cellulose-binding activities.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1111/j.1574-6968.1996.tb08177.x

Permalink

Bibliothek	Standort	Signatur	Band/Heft/Jahr	Verfügbarkeit

Andere fanden auch interessant ...

Artikel (Nationallizenzen)

Volltext

2

Digitale Medien

Demonstration of the third antigenically distinct outer membrane lipoprotein (OmlA) in Actinobacillus pleuropneumoniae serotype 7 (1998)

Ito, Hiroya ; Osaki, Makoto ; Uchida, Ikuo ; [weitere]

Oxford, UK : Blackwell Publishing Ltd

FEMS microbiology letters 167 (1998), S. 0

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 1574-6968

Quelle: Blackwell Publishing Journal Backfiles 1879-2005

Thema: Biologie

Notizen: The gene encoding an outer membrane lipoprotein (OmlA) of Actinobacillus pleuropneumoniae strain WF83 (serotype 7 reference strain), designated omlA7, was sequenced. The amino acid sequence of OmlA7 showed 64.5 and 71.6% identity to that of OmlA from serotypes 1 (OmlA1) and 5 (OmlA5), respectively. The first 134 amino acids of OmlA7 were identical to those of OmlA5. A Southern blot analysis revealed the presence of a gene highly homologous to the omlA7 in the reference strains of serotypes 3, 4, 6, and 7. A Western blot analysis using a specific antiserum against a recombinant OmlA7 detected expression of the homologous proteins in the serotypes 4, 6, and 7 reference strains and a serotype 3 field strain, but not in a serotype 3 reference strain. The data demonstrate the third antigenically distinct OmlA is expressed in A. pleuropneumoniae.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1111/j.1574-6968.1998.tb13243.x

Permalink

Bibliothek	Standort	Signatur	Band/Heft/Jahr	Verfügbarkeit

Andere fanden auch interessant ...

Artikel (Nationallizenzen)

Volltext

3

Digitale Medien

Extended Korn's inequalities and the associated best possible constants (1990)

Ito, Hiroya

Springer

Journal of elasticity 24 (1990), S. 43-78

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 1573-2681

Schlagwort(e): 35B45 ; 73C99

Quelle: Springer Online Journal Archives 1860-2000

Thema: Maschinenbau , Physik

Notizen: Abstract We are concerned with the coerciveness of the strain energy E(u) (in linear elasticity) associated with a displacement vector u on the Sobolev space H1 (Ω) or its subspaces, a domain Ω in ℝn representing an isotropic elastic body—certain specific cases are called “Korn's inequalities”. Sufficient (and necessary) conditions on the “Lamé moduli” for E(·) to be coercive (or uniformly positive) on such spaces are given, and the associated best possible constants are obtained for some cases.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00115553

Permalink

Bibliothek	Standort	Signatur	Band/Heft/Jahr	Verfügbarkeit

Andere fanden auch interessant ...

Artikel (Nationallizenzen)

Volltext

4

Digitale Medien

Best constants in Korn-Poincaré's inequalities on a slab (1994)

Ito, Hiroya

Chichester, West Sussex : Wiley-Blackwell

Mathematical Methods in the Applied Sciences 17 (1994), S. 525-549

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 0170-4214

Schlagwort(e): Mathematics and Statistics ; Applied Mathematics

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Mathematik

Notizen: We establish the best constants in the Poincaré-type and the trace-type inequalities for the quadratic form \documentclass{article}\pagestyle{empty}\begin{document}$ \lambda ||\,{\rm div}\,{\rm u}\,||_{L^2 }^2 \, + \,2\,\mu \,||\,\,(\nabla {\rm u}\, + \,\nabla {\rm u}^{\rm T})/2\,||_{L^2 }^2 $\end{document} which is fundamental in elasticity theory, on the space of H1 vector fields u on a slab vanishing on one or both of its sides. We similarly calculate those constants for the case of H1 divergence-free vector fields. Our method, which is fairly general, has another practical application to the quadratic form ∑j,k(ajk∂ku, ∂ju)L2 with coefficients a jk = akj ε C in H1 scalar functions u on a slab.

Zusätzliches Material: 6 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/mma.1670170704

Permalink

Bibliothek	Standort	Signatur	Band/Heft/Jahr	Verfügbarkeit

Andere fanden auch interessant ...

Artikel (Nationallizenzen)

Volltext