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  • 1
    Digitale Medien
    Digitale Medien
    Covalent immobilization of trypsin onto poly(2-hydroxyethyl methacrylate)/polystyrene composite microspheres by cyanogen bromide method and its enzymatic activity (1987)
    Springer
    Colloid & polymer science 265 (1987), S. 957-964 
    Details
    ISSN: 1435-1536
    Schlagwort(e): Enzymeimmobilization ; trypsin ; cyanogen bromide ; emulsion ; microsphere
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Maschinenbau
    Notizen: Abstract Covalent immobilization of trypsin onto poly(2-hydroxyethyl methacrylate)/ polystyrene composite microspheres, produced by emulsifier-free seeded emulsion polymerization technique, was carried out using the cyanogen bromide method under various conditions. The highest enzymatic activities of the trypsin immobilized in this study against N-α-benzoyl-L-arginine ethyl ester and N-α-benzoyl-L-arginine-p-nitroanilide, as low-molecular substrates and casein as a high-molecular substrate, were high, as corresponding to 80%, 85% and 50% of those of free trypsin, respectively.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01412397
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Determination of reduced-form glutathione and total glutathione in blood and plasma by high performance liquid chromatography with on-column fluorescence derivatization (1998)
    Springer
    Chromatographia 48 (1998), S. 690-694 
    Details
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; On-column fluorescence derivatization ; Blood and plasma ; Glutathione ; ortho-Phthalaldehyde
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary An on-column fluorometric derivatization method has been developed for the determination of reduced-form glutathione and total glutathione in blood and plasma by high-performance liquid chromatography. Blood hemolysate or diluted plasma was deproteinized with percholoric acid. For total glutathione determination, any oxidized forms of glutathione in samples were converted to reduced-form glutathione with dithiothreitol prior to protein precipitation. Deproteinized samples were injected without pretreatment. Glutathione was fluorescence-derivatized and separated on a C18-bonded vinyl alcohol polymer column withortho-phthalaldehyde in sodium borate buffer-acetonitrile, as mobile phase; theortho-phthalaldehyde-glutathione, fluorescent isoindole adduct is measured by its fluorescence response. Due to the high sensitivity, selectivity reproducibility and the simple HPLC system, the method is particularly suitable for routine assay of numerous or small amounts of samples.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02467600
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