Digitale Medien
Springer
Plant cell, tissue and organ culture
63 (2000), S. 121-125
ISSN:
1573-5044
Schlagwort(e):
Buddleia × weyeriana
;
colchicine
;
flow cytometry
;
micropropagation
;
ploidy
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract The aim was to produce a tetraploid form of Buddleia globosa to facilitate introgression of yellow flower colour into B. davidii, which is naturally tetraploid. Protocols were established for the micropropagation of B. globosa and tetraploid plants were obtained by application in vitro of colchicine to pre-cultured excised nodal sections. Three concentrations of colchicine were applied (0.01%, 0.05% and 0.1% w/v) for 1, 2 or 3 days. At 0.01% tetraploids were produced only after 2 days of application. All other treatments produced at least one tetraploid. The colchicine technique was extremely effective: of 29 lines tested, 19 were tetraploid and 5 were mixoploid. The vegetative characteristics of these tetraploids are described and the flowering characteristics of the three that flowered.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1023/A:1006434803003
Permalink
| Bibliothek |
Standort |
Signatur |
Band/Heft/Jahr |
Verfügbarkeit |
