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Digitale Medien

Determination of metabolites relating to the tryptophan-NAD pathway in rat urine by cellulose high performance thin-layer chromatography (1985)

Yamada, M. ; Minami, Y. ; Yuyama, S. ; [weitere]

Weinheim : Wiley-Blackwell

Journal of High Resolution Chromatography 8 (1985), S. 69-72

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Details

ISSN: 0935-6304

Schlagwort(e): Thin-layer chromatography, HPTLC ; Cellulose plates ; Tryptophan-NAD pathway ; Rat urine ; Chemistry ; Analytical Chemistry and Spectroscopy

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie

Notizen: High performance thin-layer chromatography (HPTLC) was used to determine 12 metabolites of the tryptophan-NAD pathway in rat urine. The method of separation described in the previous report was improved. The metabolites were separated on the cellulose plate by using five multiple developments with four solvent systems. The plate was scanned by the TLC scanner at 254 nm and the amounts of the metabolites were calculated by comparing peak areas of the scanning curves obtained for the urine sample and for the authentic standards. This procedure can be used as a semiquantitative determination method for the metabolites in biological fluids.

Zusätzliches Material: 1 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jhrc.1240080203

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