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Digitale Medien

Protoplast isolation in the marine brown alga Dictyopteris prolifera (Dictyotales) (1995)

Fujimura, T. ; Kawai, T. ; Kajiwara, T. ; [weitere]

Springer

Plant cell reports 14 (1995), S. 571-574

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Details

ISSN: 1432-203X

Schlagwort(e): Crassostrea gigas ; Dictyopteris prolifera ; Marine brown alga ; Protoplast ; Single cell

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract Protoplasts were isolated from thalli of Dictyopteris prolifera using a mixture of crude enzymes from vicera of live oysters (Crassostrea gigas) and the following commercial enzymes: an abalone enzyme, cellulase, polygalacturonase and hemicellulase. The enzyme mixtures produced up to 3.3 × 107 cells per l g of tissue fresh weight. The conversion to protoplasts of the cells was about 100% using the oyster enzyme or the abalone enzyme alone. The optimum pH for protoplast isolation was 6.0 and 20 hours were required for conversion to protoplasts.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00231940

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