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  • 1
    Digitale Medien
    Digitale Medien
    Gibberellic-acid causes flowering in the short-day plants Panicum miliaceum L., P. miliare lamk., and Setaria italica (L.) P. Beauv. (1977)
    Springer
    Planta 134 (1977), S. 95-96 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Flowering ; Gibberellin ; Panicum ; Photoperiod ; Setaria
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Treatment with gibberellic acid (GA3) causes formation of flowers in Panicum ciliaceum and Panicum miliare, two short-day plants, under long days (continuous light), and hastens the emergence of ears in Setaria italica, a quantitative short-day plant, under both inductive and non-inductive photoperiods. The GA3-induced inflorescences, however, remain short and bear only few spikelets; in the two Panicum species, the spikelets also remain sterile.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00390101
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Agrobacterium-mediated engineering for sheath blight resistance of indica rice cultivars from different ecosystems (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 832-839 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Agrobacterium ; Chitinase ; T-DNA ; Sheath blight ; Rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  A concise T-DNA element was engineered containing the rice class-I chitinase gene expressed under the control of CaMV35S and the hygromycin phosphotransferase gene (hph) as a selectable marker. The binary plasmid vector pNO1 with the T-DNA element containing these genes of interest was mobilized to Agrobacterium tumefaciens strain LBA4404 to act as an efficient donor of T-DNA in the transformation of three different indica rice cultivars from different ecosystems. Many morphologically normal, fertile transgenic plants from these rice cultivars were generated after Agrobacterium-mediated transformation using 3-week-old scutella calli as initial explants. Stable integration, inheritance and expression of the chimeric chitinase gene were demonstrated by Southern blot and Western blot analysis of the transformants. Bioassay data showed that transgenic plants can restrict the growth of the sheath blight pathogen Rhizoctonia solani. Bioassay results were correlated with the molecular analysis. Although we obtained similar results upon DNA-mediated transformation, this report shows the potential of the cost-effective, simple Agrobacterium system for genetic manipulation of rice cultivars with a pathogenesis-related (PR) gene.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051359
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